M4038
SigmaMarker™
Wide Range, mol wt 6,500-205,000 Da
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About This Item
UNSPSC Code:
12352200
MDL number:
form
lyophilized powder
usage
sufficient for 10-20 large gels (16 × 18 cm), sufficient for 20-30 mini-gels (10 × 10 cm), vial sufficient for 50-100 PhastGels
mol wt
6,500-205,000 Da
storage temp.
2-8°C
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Application
SigmaMarkers are designed for use with Laemmli SDS-PAGE, the PhastSystem (Pharmacia) and Schagger and von Jagow SDS-PAGE (Tricine). SigmaMarkers cover the range of molecular weights common to most proteins or their subunits. The wide, high and low range markers are lyophilized with sample buffer so that they are ready-to-use after reconstitution with deionized water. The markers are formulated to yield a distribution of well-defined bands of approximately equal intensity after electrophoresis and Coomassie blue staining.
Preparation Note
Reconstitution with 100 μl of deionized water results in a solution containing 2-3.5 mg of protein per ml of 62 mM Tris-HCl, pH 6.8, 1 mM EDTA, 4% sucrose, 0.5% dithiothreitol, 2% SDS, and 0.005% bromophenol blue.
Other Notes
Will yield 13 protein bands with Coomassie Blue staining: aprotinin, α-lactalbumin, trypsin inhibitor, trypsinogen, carbonic anhydrase, glyceraldehyde-3-phosphate dehydrogenase, ovalbumin, glutamic dehydrogenase, albumin, fructose-6-phosphate kinase, phosphorylase b, β-galactosidase and myosin
Legal Information
SigmaMarker is a trademark of Sigma-Aldrich Co. LLC
Regulatory Information
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