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M3942

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DMEM - high glucose

MegaCell™, with sodium pyruvate, without ʟ-glutamine, sterile liquid, suitable for cell cutlure

Synonym(s):

DMEM, DMEM Supplement

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

product name

MegaCell™ Dulbecco′s Modified Eagle′s Medium, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

Quality Level

300

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: no
glucose: 4500 mg/L (Dextro)
sodium pyruvate: yes

shipped in

ambient

storage temp.

2-8°C

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General description

MegaCell™ is a multifaceted media developed to reduce the amount of serum required for culturing mammalian cells in vitro. It reduces the supplementation of fetal bovine serum (FBS) or other serum from 10% to 3% and supports proliferative rates and maximal cell densities. MegaCell enables maintaining a long-term culture of non-fastidious cell lines.

Application

MegaCell™ Dulbecco′s Modified Eagle′s Medium has been used:
  • as a component of the M10 medium to culture CD133+ cells from biopsies of para-spinal and quadriceps muscle of Duchenne muscular dystrophy patients
  • as a component of the proliferation medium for the expansion and cryopreservation of human induced pluripotent stem (iPS) cells cultured without feeder cells and human embryonic (ES) cell-derived mesoangioblast-like cells
  • to culture the positive hybridomas during the production of mouse anti-chicken CD25 monoclonal antibodies

Reconstitution

Supplement with 4 mM L-glutamine (20 ml of 200 mM solution, G7513)

Legal Information

Sigma-Aldrich uses the MegaCell trademark pursuant to an agreement with Promega Corporation.
MegaCell is a trademark of Promega Corp.

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supplement

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WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

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Shintaro Iwashita et al.
Bioscience reports, 35(4), doi:10-doi:10 (2015-07-17)
The BCNT (Bucentaur) superfamily is classified by an uncharacteristic conserved sequence of ∼80 amino acids (aa) at the C-terminus, BCNT-C (the conserved C-terminal region of Bcnt/Cfdp1). Whereas the yeast Swc5 and Drosophila Yeti homologues play crucial roles in chromatin remodelling

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