M3942
DMEM - high glucose
MegaCell™, with sodium pyruvate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell cutlure
Synonym(s):
DMEM, DMEM Supplement
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
Product Name
MegaCell™ Dulbecco′s Modified Eagle′s Medium, liquid, sterile-filtered, suitable for cell culture
sterility
sterile-filtered
Quality Segment
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
L-glutamine: no
glucose: 4500 mg/L (Dextro)
sodium pyruvate: yes
shipped in
ambient
storage temp.
2-8°C
General description
MegaCell™ is a multifaceted media developed to reduce the amount of serum required for culturing mammalian cells in vitro. It reduces the supplementation of fetal bovine serum (FBS) or other serum from 10% to 3% and supports proliferative rates and maximal cell densities. MegaCell enables maintaining a long-term culture of non-fastidious cell lines.
Application
MegaCell™ Dulbecco′s Modified Eagle′s Medium has been used:
- as a component of the M10 medium to culture CD133+ cells from biopsies of para-spinal and quadriceps muscle of Duchenne muscular dystrophy patients
- as a component of the proliferation medium for the expansion and cryopreservation of human induced pluripotent stem (iPS) cells cultured without feeder cells and human embryonic (ES) cell-derived mesoangioblast-like cells
- to culture the positive hybridomas during the production of mouse anti-chicken CD25 monoclonal antibodies
Preparation Note
Supplement with 4 mM L-glutamine (20 ml of 200 mM solution, G7513)
Legal Information
Sigma-Aldrich uses the MegaCell trademark pursuant to an agreement with Promega Corporation.
MegaCell is a trademark of Promega Corp.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
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