M3788
SigmaMarker™
High Range, mol wt 36,000-205,000 Da
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About This Item
UNSPSC Code:
12352200
MDL number:
form
lyophilized powder
usage
sufficient for 10-20 large gels (16 × 18 cm), sufficient for 20-30 mini-gels (10 × 10 cm), vial sufficient for 50-100 PhastGels
mol wt
36,000-205,000 Da
storage temp.
2-8°C
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Application
SigmaMarkers are designed for use with Laemmli SDS-PAGE, the PhastSystem (Pharmacia) and Schagger and von Jagow SDS-PAGE (Tricine). SigmaMarkers cover the range of molecular weights common to most proteins or their subunits. The wide, high and low range markers are lyophilized with sample buffer so that they are ready-to-use after reconstitution with deionized water. The markers are formulated to yield a distribution of well-defined bands of approximately equal intensity after electrophoresis and Coomassie blue staining.
Preparation Note
Reconstitution with 100 μl of deionized water results in a solution containing 2-3.5 mg of protein per ml of 62 mM Tris-HCl, pH 6.8, 1 mM EDTA, 4% sucrose, 0.5% dithiothreitol, 2% SDS, and 0.005% bromophenol blue.
Other Notes
Will yield eight protein bands with Coomassie Blue staining: glyceraldehyde-3-phosphate dehydrogenase, ovalbumin, glutamic dehydrogenase, albumin, fructose-6-phosphate kinase, phosphorylase b, β-galactosidase and myosin.
Legal Information
SigmaMarker is a trademark of Sigma-Aldrich Co. LLC
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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