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M2820

Sigma-Aldrich

Anti-MSH6 (C-terminal) antibody produced in rabbit

~1 μg/L, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Colorectal cancer, hereditary noopolyposis, type 5, included, Anti-G/T Mismatch-binding protein, Anti-GTBP, Anti-HNPCC5, Anti-mutS (E. coli) homolog 6

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About This Item

MDL number:
MFCD09039195
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 160 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

concentration

~1 μg/L

technique(s)

immunoprecipitation (IP): 2.5-5 μg using HEK 293T cell lysates
indirect immunofluorescence: 10-20 μg/mL using paraformaldehyde-fixed HeLa cells
microarray: suitable
western blot: 0.5-1 μg/mL using HEK 293T cell lysates

UniProt accession no.

P52701

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MSH6(2956)

General description

MutS Homolog 6 (MSH6) is localized on human chromosome 2p16.3. MSH6 is a 160 kDa protein with nuclear localization sequence and a N-terminal disordered domain. It is a human homolog of E. coli MutS.

Immunogen

synthetic peptide corresponding to amino acids 1311-1327 of human MSH6, conjugated to KLH via an N-terminal added cysteine residue. The immunizing peptide differs from the rat and mouse sequences in one amino acid.

Application

Anti-MSH6 (C-terminal) antibody produced in rabbit has been used in:
  • immunoblotting
  • immunoprecipitation
  • immunofluorescence

Biochem/physiol Actions

MSH6 (also known as GTBP) was isolated from HeLa cells by virtue of its ability to restore mismatch repair to nuclear extracts of MSH2-deficient colorectal tumor cell lines. MutS Homolog 6 (MSH6) is part ofDNA repair pathways and play a key role onmutator S protein (hMutSα) shuttling. MSH6 has ATPase activity that is critical in mismatch repair. Germline mutations in MSH6 is associated with hereditary nonpolyposis colon cancer and in endometrial carcinoma.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Certificates of Analysis (COA)

Lot/Batch Number
068M4785
106K4789

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Structural, molecular and cellular functions of MSH2 and MSH6 during DNA mismatch repair, damage signaling and other noncanonical activities
Edelbrock MA, et al.
Mutation Research. Fundamental and Molecular Mechanisms of Mutagenesis, 743, 53-66 (2013)
Prevalence of defective DNA mismatch repair and MSH6 mutation in an unselected series of endometrial cancers
Goodfellow PJ, et al.
Proceedings of the National Academy of Sciences of the USA, 100(10), 5908-5913 (2003)
Inhibition of Msh6 ATPase activity by mispaired DNA induces a Msh2 (ATP)-Msh6 (ATP) state capable of hydrolysis-independent movement along DNA
Mazur DJ
Molecular Cell, 22(1), 39-49 (2006)

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