M2414
Minimum Essential Medium Eagle
With Earle′s salts and reduced NaHCO₃. without ʟ-glutamine, sterile liquid, suitable for cell culture
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Synonym(s):
EMEM, MEM
UNSPSC Code:
12352207
NACRES:
NA.75
Recommended Products
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
phenol red: 0.011 g/L
NaHCO3: 0.85 g/L
glucose: 1.0 g/L
L-glutamine: no
shipped in
ambient
storage temp.
2-8°C
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General description
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.
MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.
MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.
Application
Recommended for use in a carbon dioxide-free culture system.
Other Notes
This product lacks L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser and L-Gln.
May also be supplemented with HEPES to raise the buffering range.
Reconstitution
Supplement with 0.292 g/L L-glutamine.
also commonly purchased with this product
Product No.
Description
Pricing
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
动植物来源培养基
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Articles
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
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