M176
M3 Muscarinic Receptor human
recombinant, expressed in Sf9 cells, membrane suspension
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About This Item
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recombinant
expressed in Sf9 cells
form
membrane suspension
packaging
vial of 50 units
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... CHRM3(1131)
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Preparation Note
Membrane suspension in 50 mM Tris-HCl, pH 7.4, 10% glycerol, and 1% BSA.
Analysis Note
Recommended assay conditions are supplied on the product data sheet. This is a bioassayed product.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Molecular pharmacology, 40(5), 783-789 (1991-11-01)
A synthetic oligopeptide (QCDKRKRRKQQYQQRQSV) corresponding to a carboxyl-terminal sequence of the rat m3 receptor (amino acids 561-578) was coupled to carrier proteins and used to generate a polyclonal antiserum. This serum selectively immunoprecipitates at least 90% of the m3 receptors
The Journal of biological chemistry, 268(13), 9817-9823 (1993-05-05)
A specific antiserum against the human m3-muscarinic receptor subtype was made by subcloning a variant region of the third intracellular loop of the m3-receptor (Ser345-Leu463) into a bacterial expression plasmid that produced a fusion protein with glutathione S-transferase. In immunoblot
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