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Key Documents

Safety Information

M176

Sigma-Aldrich

M3 Muscarinic Receptor human

recombinant, expressed in Sf9 cells, membrane suspension

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About This Item

MDL number:
UNSPSC Code:
12352200

recombinant

expressed in Sf9 cells

form

membrane suspension

packaging

vial of 50 units

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... CHRM3(1131)

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Preparation Note

Membrane suspension in 50 mM Tris-HCl, pH 7.4, 10% glycerol, and 1% BSA.

Analysis Note

Recommended assay conditions are supplied on the product data sheet. This is a bioassayed product.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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S J Wall et al.
Molecular pharmacology, 40(5), 783-789 (1991-11-01)
A synthetic oligopeptide (QCDKRKRRKQQYQQRQSV) corresponding to a carboxyl-terminal sequence of the rat m3 receptor (amino acids 561-578) was coupled to carrier proteins and used to generate a polyclonal antiserum. This serum selectively immunoprecipitates at least 90% of the m3 receptors
A B Tobin et al.
The Journal of biological chemistry, 268(13), 9817-9823 (1993-05-05)
A specific antiserum against the human m3-muscarinic receptor subtype was made by subcloning a variant region of the third intracellular loop of the m3-receptor (Ser345-Leu463) into a bacterial expression plasmid that produced a fusion protein with glutathione S-transferase. In immunoblot

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