All Photos(6)
Synonym(s):
3-(N-Morpholino)propanesulfonic acid, 4-Morpholinepropanesulfonic acid
Empirical Formula (Hill Notation):
C7H15NO4S
CAS Number:
Molecular Weight:
209.26
Beilstein:
1106776
EC Number:
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.25
Recommended Products
Quality Level
Assay
≥99.5% (titration)
form
crystalline powder
storage condition
dry at room temperature
technique(s)
affinity chromatography: suitable
electrophoresis: suitable
color
white
pH
2.5-4 (25 °C, 209 g/L)
useful pH range
6.5-7.9
pKa (25 °C)
7.2
solubility
water: 0.5 g/mL, clear, colorless
λ
33 % in H2O
suitability
suitable for HPLC
application(s)
clinical research
diagnostic assay manufacturing
life science and biopharma
storage temp.
room temp
SMILES string
OS(=O)(=O)CCCN1CCOCC1
InChI
1S/C7H15NO4S/c9-13(10,11)7-1-2-8-3-5-12-6-4-8/h1-7H2,(H,9,10,11)
InChI key
DVLFYONBTKHTER-UHFFFAOYSA-N
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General description
MOPS (3-(N-morpholino)propanesulfonic acid) also known as Propane Sultone buffer, is a versatile "Good′s buffer" extensively employed in biological research. It shares structural similarities with MES (2-(N-morpholino)ethanesulfonic acid), featuring a morpholine ring. In terms of physiological relevance, MOPS′ pKa of 7.2 closely aligns with physiological pH, making it suitable for studies requiring a near-neutral environment. Compared to MES with a pKa of 6.1, MOPS offers a more physiologically relevant buffering capacity.
MOPS serves various functions in diverse research domains. As a buffering agent, it can be used to stabilize pH in cell culture media, promoting optimal cell growth and function. Additionally, it may find application in protein purification in chromatography, stabilizes protein solutions, and acts as a reliable running buffer in various electrophoretic techniques, enabling efficient separation and analysis of biomolecules. MOPS also efficiently lyses cells for subsequent protein or nucleic acid extraction and stabilizes enzymes in solution, ensuring their activity for research purposes. It further proves valuable in fine-tuning the pH of growth media for optimal cell growth.
MOPS possesses additional advantages such as minimal interaction with metal ions especially with copper (Cu), nickel (Ni), manganese (Mn), zinc (Zn), cobalt (Co) ions, making it ideal for studies involving metal ions. It exhibits excellent water solubility, facilitating its use in various buffer formulations, and minimal lipid solubility, ensuring impermeability to membranes and minimizing interference with cellular processes. In summary, MOPS is a reliable buffer widely utilized across diverse biological disciplines, distinguished by its versatility, physiological relevance, minimal metal interaction, and ease of use, with its extensive applications in cell biology, protein studies, electrophoresis, and biochemical research.
MOPS serves various functions in diverse research domains. As a buffering agent, it can be used to stabilize pH in cell culture media, promoting optimal cell growth and function. Additionally, it may find application in protein purification in chromatography, stabilizes protein solutions, and acts as a reliable running buffer in various electrophoretic techniques, enabling efficient separation and analysis of biomolecules. MOPS also efficiently lyses cells for subsequent protein or nucleic acid extraction and stabilizes enzymes in solution, ensuring their activity for research purposes. It further proves valuable in fine-tuning the pH of growth media for optimal cell growth.
MOPS possesses additional advantages such as minimal interaction with metal ions especially with copper (Cu), nickel (Ni), manganese (Mn), zinc (Zn), cobalt (Co) ions, making it ideal for studies involving metal ions. It exhibits excellent water solubility, facilitating its use in various buffer formulations, and minimal lipid solubility, ensuring impermeability to membranes and minimizing interference with cellular processes. In summary, MOPS is a reliable buffer widely utilized across diverse biological disciplines, distinguished by its versatility, physiological relevance, minimal metal interaction, and ease of use, with its extensive applications in cell biology, protein studies, electrophoresis, and biochemical research.
Application
MOPS has been used as:
- a cell culture additive component in lentiviral particle production
- as a buffering agent in microbial growth medium and nuclei extraction buffer
- as a component of the MMSE-A buffer for isolating heart mitochondria
- as a component of the Synthetic Complete (SC) medium
- as a buffer in fluorescence quenching of NADH
Features and Benefits
- Ideal for Cell Biology and Biochemical research
- Can be used as a Buffer component for Electrophoresis and Protein separation
- Effective Buffering from pH 6.5-7.9 (25 °C) with a pKa of 7.2 (25 °C)
- Highly soluble in water
- Minimal metal ion binding
Other Notes
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WGK
WGK 1
Flash Point(F)
closed cup
Flash Point(C)
closed cup
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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(Un) suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions-a review
Ferreira CMH, et al.
Royal Society of Chemistry Advances, 5(39), 30989-31003 (2015)
Interactions of biological buffers with macromolecules: The ubiquitous ?smart? polymer PNIPAM and the biological buffers MES, MOPS, and MOPSO
Taha M, et al.
Macromolecules, 44(21), 8575-8589 (2011)
PROTOCOLS: Chromatin immunoprecipitation from Arabidopsis tissues
Yamaguchi N, et al.
The Arabidopsis Book / American Society of Plant Biologists, 12(3), e170-e170 (2014)
Survival to different acid challenges and outer membrane protein profiles of pathogenic Escherichia coli strains isolated from pozol, a Mexican typical maize fermented food
Sainz T, et al.
International Journal of Food Microbiology, 105(3), 357-367 (2005)
Junji Suzuki et al.
Nature communications, 5, 4153-4153 (2014-06-14)
The endoplasmic reticulum (ER) and mitochondria accumulate Ca(2+) within their lumens to regulate numerous cell functions. However, determining the dynamics of intraorganellar Ca(2+) has proven to be difficult. Here we describe a family of genetically encoded Ca(2+) indicators, named calcium-measuring
Protocols
Overview of polyacrylamide gel chemistry and detailed instructions for hand-casting polyacrylamide gels using mPAGE® TurboMix Bis-Tris Gel Casting Kits.
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