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M042030

Sigma-Aldrich

Biotin Phosphoramidite

configured for ABI

Synonym(s):

Biotin amidite

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About This Item

Empirical Formula (Hill Notation):
C46H64N5O8PS
CAS Number:
1217500-22-5
Molecular Weight:
878.07
MDL number:
MFCD00676535
UNSPSC Code:
41116105
PubChem Substance ID:
329817780
NACRES:
NA.21

product line

Proligo Reagents

Quality Level

200

Assay

≥96% (31P-NMR)

suitability

conforms to structure for H-NMR

compatibility

configured for ABI

storage temp.

−20°C

SMILES string

COc1ccc(cc1)C(N2C3CS[C@@H](CCCCC(=O)NCCOCCOCCOP(OCCC#N)N(C(C)C)C(C)C)C3NC2=O)(c4ccccc4)c5ccc(OC)cc5

InChI

1S/C46H64N5O8PS/c1-34(2)51(35(3)4)60(58-27-12-25-47)59-32-31-57-30-29-56-28-26-48-43(52)16-11-10-15-42-44-41(33-61-42)50(45(53)49-44)46(36-13-8-7-9-14-36,37-17-21-39(54-5)22-18-37)38-19-23-40(55-6)24-20-38/h7-9,13-14,17-24,34-35,41-42,44H,10-12,15-16,26-33H2,1-6H3,(H,48,52)(H,49,53)/t41-,42-,44-,60?/m0/s1

InChI key

WSDQYLGSIUJJRU-ZMKGMYJVSA-N

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General description

Biotin phosphoramidite is a reagent used to directly incorporate a biotin label at the 5′ hydroxy group of the terminal nucleoside base through a p-aminophenethyl spacer group during oligonucleotide synthesis. Biotin-labelled oligonucleotides have become extremely popular in DNAcapture and detection applications, due to their very high-sensitivity and strong, specific binding affinity with avidin and streptavidin proteins. Biotinbased detection methods and are widely used by researchers. Biotin labelling is compatible with PCR and most hybridization techniques.

Key Features of Biotin Phosphoramidite

  • Behaves like any standard phosphoramidite on the DNA synthesizer
  • Readily soluble in acetonitrile
  • Biotin compound has a dimethoxytrityl (DMT) group on the ring structure,which allows coupling-yield determination and trityl-selective purification
  • A long, water-compatible linker arm ensures maximum sensitivity
  • Provides a coupling efficiency of at least 95%The product is configured for ABI™ Synthesizers

Legal Information

ABI is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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