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M0194

Sigma-Aldrich

Anti-Muscarinic Acetylcholine Receptor (M3) antibody produced in rabbit

affinity isolated antibody, lyophilized powder

Synonym(s):

Muscarinic Acetylcholine Receptor M3 Antibody, Muscarinic Acetylcholine Receptor M3 Antibody - Anti-Muscarinic Acetylcholine Receptor (M3) antibody produced in rabbit

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About This Item

MDL number:
MFCD06798471
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

mouse, rat, human

technique(s)

western blot: 1:200 using rat brain lysate

UniProt accession no.

P20309

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CHRM3(1131)
mouse ... Chrm3(12671)
rat ... Chrm3(24260)

General description

Cholinergic receptor muscarinic 3 (CHRM3) gene is located on the human chromosome at 1q43 and encodes for the M3 subtype of muscarinic acetylcholine (ACh) receptors. Muscarinic receptor 3 (M3R) is one of the five muscarinic receptors (M1R-M5R). It is a member of the class A family of G-protein coupled receptors (GPCRs). M3R is localized to the salivary glands, lachrymal glands, trachea and bronchi.

Specificity

Rabbit polyclonal anti-Muscarinic Acetylcholine Receptor (M3) antibody recognizes rat, human, mouse Muscarinic Acetylcholine Receptor (M3).

Immunogen

synthetic peptide corresponding to amino acid residues 461-479 of the third intracellular loop of rat muscarinic acetylcholine receptor M3. This sequence is identical in human, mouse, pig, and bovine.

Application

Anti-Muscarinic Acetylcholine Receptor (M3) antibody produced in rabbit has been used in immunohistochemistry and western blotting.
Rabbit polyclonal anti-Muscarinic Acetylcholine Receptor (M3) antibody is used to tag muscarinic acetylcholine receptor (M3) for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of muscarinic acetylcholine receptor (M3) in information processing and plastic changes in CNS function.

Biochem/physiol Actions

Muscarinic receptor 3 (M3R) is involved in the contraction of the detrusor muscle. It is also involved in the secretion of saliva and bladder contraction. It participates in several pathological processes of lung disease, such as chronic obstructive pulmonary disease (COPD). Mutations in the CHRM3 gene is associated with congenital bladder malformations related to prune-belly-like syndrome (PBS).

Physical form

Lyophilized from phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictograms

Skull and crossbones
GHS06

Signal Word

Danger

Hazard Statements

H302 + H332,H311,H412

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品
含少量动物源组分生物产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Muscarinic receptor subtypes and management of the overactive bladder
Chapple C R, et al.
Urology, 60(5), 82-88 (2002)
Novel insights into M 3 muscarinic acetylcholine receptor physiology and structure
Kruse A C, et al.
Journal of Molecular Neuroscience, 53(3), 316-323 (2014)
Chapter 4 - Cholinergic Signaling and Muscle Contraction
Signal Transduction, 11(4), 263-327 (2016)
Overexpression of muscarinic receptor 3 promotes metastasis and predicts poor prognosis in non-small-cell lung cancer
Lin G, et al.
Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer, 9(2), 170-178 (2014)
Muscarinic acetylcholine receptor M3 mutation causes urinary bladder disease and a prune-belly-like syndrome
Weber S, et al.
American Journal of Human Genetics, 89(5), 668-674 (2011)
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