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L9125

Sigma-Aldrich

L-Leucine-p-nitroanilide

leucine aminopeptidase substrate, chromogenic, ≥98% (TLC), powder

Synonym(s):

L-Leucine-4-nitroanilide

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About This Item

Empirical Formula (Hill Notation):
C12H17N3O3
CAS Number:
4178-93-2
Molecular Weight:
251.28
Beilstein:
2218433
EC Number:
224-047-3
MDL number:
MFCD00063681
UNSPSC Code:
12352204
PubChem Substance ID:
24896516
NACRES:
NA.32

product name

L-Leucine-p-nitroanilide, leucine aminopeptidase substrate

Quality Level

200

Assay

≥98% (TLC)

form

powder

solubility

ethanol: 50 mg/mL

storage temp.

2-8°C

SMILES string

CC(C)C[C@H](N)C(=O)Nc1ccc(cc1)[N+]([O-])=O

InChI

1S/C12H17N3O3/c1-8(2)7-11(13)12(16)14-9-3-5-10(6-4-9)15(17)18/h3-6,8,11H,7,13H2,1-2H3,(H,14,16)/t11-/m0/s1

InChI key

AXZJHDNQDSVIDR-NSHDSACASA-N

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Application

L-Leucine-p-nitroanilide has been used as substrate
  • for the determination of intestinal leucine aminopeptidase activity of juvenile rainbow trout (Oncorhynchus mykiss)
  • in insulin-regulated aminopeptidase inhibition assay
  • for determining exopeptidase activity

Substrates

Substrate for the colorimetric determination of leucine aminopeptidase.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317,H319

Hazard Classifications

Eye Irrit. 2 - Skin Sens. 1

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A Bernkop-Schnürch et al.
Pharmaceutical research, 14(2), 181-185 (1997-02-01)
Develop and evaluate systems to prevent aminopeptidase N caused enzymatic degradation of perorally administrated peptide drugs. Bacitracin was covalently bound to the unabsorbable carrier matrix poly(acrylic acid) (paa) in order to avoid any dilution effects of the inhibitor in the
Effects of fish meal replacement with meat and bone meal using garlic (Allium sativum) powder on growth, feeding, digestive enzymes and apparent digestibility of nutrients and fatty acids in juvenile rainbow trout (Oncorhynchus mykiss Walbaum, 1792)
Esmaeili M, et al.
Aquaculture Nutrition, 23(6), 1225-1234 (2017)
Identification of drug-like inhibitors of insulin-regulated aminopeptidase through small-molecule screening
Engen K, et al.
Assay and Drug Development Technologies, 14(3), 180-193 (2016)
W Bawab et al.
The Biochemical journal, 286 ( Pt 3), 967-975 (1992-09-15)
Aminopeptidase activities were identified in extracts of kidney, ovotestis, head ganglia, heart and haemolymph of Aplysia californica. These enzyme preparations hydrolysed [3H][Leu]enkephalin at the Try-1-Gly-2 bond as determined by h.p.l.c. analysis of cleavage products. In all these tissues, enkephalin-degrading aminopeptidase
Reishi Toshiyama et al.
Oncogene, 38(2), 244-260 (2018-08-10)
Previous studies highlighted that aminopeptidase N (APN)/CD13 acts as a scavenger in the survival of hepatocellular carcinoma (HCC) stem cells by reducing reactive oxygen species (ROS) levels. Hence, it has been proposed that APN/CD13 inhibition can increase cellular ROS levels
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