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L8777

Sigma-Aldrich

Lectin from Phytolacca americana (pokeweed)

lyophilized powder, BioReagent, suitable for cell culture

Synonym(s):

PWM, Pokeweed mitogen

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Select a Size

25 G
CN¥852.84
100 G
CN¥861.16
500 G
CN¥3,092.76
1 KG
CN¥7,152.31
10 KG
CN¥33,034.01

CN¥852.84

Available to ship onApril 25, 2025Details

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25 G
CN¥852.84
100 G
CN¥861.16
500 G
CN¥3,092.76
1 KG
CN¥7,152.31
10 KG
CN¥33,034.01

About This Item

MDL number:
MFCD00161973
UNSPSC Code:
12352202
NACRES:
NA.32

CN¥852.84

Available to ship onApril 25, 2025Details

sterility

aseptically filled

Quality Level

200

product line

BioReagent

form

lyophilized powder

potency

≤0.3 μg/mL mitogenic activity

technique(s)

cell culture | mammalian: suitable

solubility

PBS: 1 mg/mL

storage temp.

2-8°C

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This Item
117458169101174580891011685597910
生物素标记缺口转录混合物 suitable for hybridization, solution, sufficient for 40 labeling reactions, pkg of 160 μL

11745824910

生物素标记缺口转录混合物

DIG-切口平移混合物 sufficient for 40 labeling reactions, pkg of 160 μL, suitable for hybridization

11745816910

DIG-切口平移混合物

缺口转录混合物 sufficient for 50 labeling reactions, pkg of 200 μL, solution

11745808910

缺口转录混合物

生物素RNA标记混合物 sufficient for 20 reactions (transcription), pkg of 40 μL, solution

11685597910

生物素RNA标记混合物

technique(s)

hybridization: suitable

technique(s)

hybridization: suitable

technique(s)

nucleic acid labeling: suitable

technique(s)

-

form

solution

form

solution

form

solution

form

solution

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

usage

sufficient for 40 labeling reactions

usage

sufficient for 40 labeling reactions

usage

sufficient for 50 labeling reactions

usage

sufficient for 20 reactions (transcription)

packaging

pkg of 160 μL

packaging

pkg of 160 μL

packaging

pkg of 200 μL

packaging

pkg of 40 μL

General description

Lectins are proteins of plant origin, which have the ability to bind to certain neuronal surface glycoproteins and glycolipids. This ability of theirs helps them to be used as neuroanatomical tracers, as they are taken and transported in a retrograde or anterograde fashion.[1]

Application

Lectin from Phytolacca americana (pokeweed) is used for proliferation Assay. (Cellular proliferative responses were measured by the incorporation of 3H thymidine in newly synthesized DNA, using a conventional assay).[2]

Biochem/physiol Actions

Lectins are carbohydrate-binding proteins and are highly specific for sugar moieties. Pokeweed lectin (PL) is a lectin specific for N-acetylglucosamine-containing saccharides that stimulate peripheral lymphocytes to undergo mitosis by binding to their cell surfaces. Four types of lectins are isolated from the roots of pokeweed (Phytolacca americana). They contain homologous domains but have different molecular sizes and biological properties.[3]
PWM is extracted from pokeweed roots. This protein has hemagglutinating, leukoagglutinating, and mitogenic properties. PWM is not blood group specific, but has affinity for N-acetyl-β-D-glucosamine oligomers.

Quality

Partially purified TCA precipitate.
Essentially salt-free.

Analysis Note

Mitogenic activity was determined by BrdU incorporation in HeLa cell cultures.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Precautionary Statements

P261 - P280 - P342 + P311

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

动植物源性产品
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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000433584
    0000433584
    0000427224
    0000427224
    0000428558

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    Kurt Hanevik et al.
    The Journal of infectious diseases, 204(11), 1779-1786 (2011-10-13)
    Clinical and epidemiological studies have suggested the development of acquired immunity in individuals previously infected with Giardia lamblia. However, there are no data on the long-term cellular immunity and genotype cross-reactivity. An outbreak of assemblage B giardiasis in a nonendemic
    Caroline Leeth et al.
    Animals : an open access journal from MDPI, 11(10) (2021-10-24)
    Immunological studies in the horse are frequently hampered by lack of environmental control, complicated study design, and ethical concerns when performing high risk studies. The purpose of the current study was to investigate the utility of a xenograft model for
    Tomomi Fujii et al.
    Acta crystallographica. Section D, Biological crystallography, 60(Pt 4), 665-673 (2004-03-25)
    Pokeweed lectin (PL), a lectin specific for N-acetylglucosamine-containing saccharides, stimulates peripheral lymphocytes to undergo mitosis by binding to their cell surfaces. Four types of lectins have been isolated from the roots of pokeweed (Phytolacca americana) and shown to contain homologous
    Samira Rezaei et al.
    Scientific reports, 10(1), 7471-7471 (2020-05-06)
    In the routine commercial karyotype analysis on 5,481 boars, we identified 32 carriers of mosaic reciprocal translocations, half of which were carrying a specific recurrent translocation, mos t(7;9). An additional 7 mosaic translocations were identified through lymphocyte karyotype analysis from
    C R Gerfen et al.
    Brain research, 290(2), 219-238 (1984-01-09)
    A new neuroanatomical method for tracing connections in the central nervous system based on the anterograde axonal transport of the kidney bean lectin, Phaseolus vulgaris-leucoagglutinin (PHA-L) is described. The method, for which a detailed protocol is presented, offers several advantages
    View All Related Papers

    Articles

    Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

    Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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