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Safety Information

L6274

Sigma-Aldrich

Laminin from human placenta

liquid, BioReagent, suitable for cell culture

Synonym(s):

Cell culture grade laminin, Human Laminin

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About This Item

CAS Number:
114956-81-9
MDL number:
MFCD00081739
UNSPSC Code:
12352202
NACRES:
NA.77

biological source

human placenta

Quality Level

300

product line

BioReagent

form

liquid

concentration

0.5 mg/mL in Tris-buffered saline

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑2 μg/cm2

UniProt accession no.

P07942

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... LAMB1(3912)

Application

Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.

Biochem/physiol Actions

Laminin proteins are integral components of structural scaffolding in animal tissues. They associate with type IV collagen via entactin and perlecan and bind to cell membranes through integrin receptors, dystroglycan glycoprotein complexes and Lutheran blood group glycoproteins. Laminin has active domains for collagen binding, cell adhesion, heparin binding, and neurite outgrowth fragment.

Components

Laminin is an extracellular matrix multidomain trimeric glycoprotein, and is the main non-collagenous component of basal lamina that supports adhesion, proliferation and differentiation. Laminin is composed of both A, B1 and B2 chains, which are connected by many disulfide bonds. This laminin product is purified from human placenta using pepsin treatment and immunoaffinity chromatography. SDS-PAGE results in two major bands at 50 kDa and 130-160 kDa.

Caution

It is recommended to store this product at -70°C, where it will remain stable for two years.

Preparation Note

This product is supplied at a concentration of 0.5 mg/mL in 50 mM Tris-HCl, with 150 mM NaCl. It is 0.2 μm filtered. Thaw this solution slowly before use at 2-8°C to avoid gel formation. For use as a coating, dilute in a sterile balanced salt solution, coat culture surface with a minimal volume and incubate at 37°C for two hours. Wash 3 times with PBS before plating cells. Laminin coatings can be stored for one month at 2-8°C.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. How do you prepare Product L6274, Laminin from human placenta?

    To prepare a laminin solution, we recommend that the L6274 be thawed at 2-8°C to avoid the formation of a gel. This can be further diluted in a balanced salt solution. If the product has been thawed at room temperature and ends up as a gel, it cannot be reactivated for use.

  6. How long can cover slips coated with Product L6274, Laminin from human placenta, be stored?

    Laminin coatings, such as laminin coated cover slips last about one month at 2-8°C. Do not use the product if discoloration or spider web formations appear on the surface of the coated material.

  7. Is Product L6274, Laminin from human placenta, laminin 1 or 2?

    Fifteen laminin trimers have been identified. We do not determine what type of laminin this is. Typically laminin from placenta is considered laminin-2/4 and laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane is laminin-1.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Satu Marja Myllymäki et al.
PloS one, 6(5), e19453-e19453 (2011-05-17)
Formation of apical compartments underlies the morphogenesis of most epithelial organs during development. The extracellular matrix (ECM), particularly the basement membrane (BM), plays an important role in orienting the apico-basal polarity and thereby the positioning of apical lumens. Integrins have
Danielle K Lewis et al.
Aging cell, 7(6), 836-849 (2008-09-10)
Astrocytes comprise a large proportion of the central nervous system support cells and play a critical role in neural injury and repair. The present study examined the impact of ovarian aging using an ex vivo model system, where astrocytes were
Terhi P Teräväinen et al.
PloS one, 8(8), e71485-e71485 (2013-08-27)
The properties of epithelial cells within tissues are regulated by their immediate microenvironment, which consists of neighboring cells and the extracellular matrix (ECM). Integrin heterodimers orchestrate dynamic assembly and disassembly of cell-ECM connections and thereby convey biochemical and mechanical information
Kelly E Fathers et al.
The American journal of pathology, 176(1), 446-460 (2009-12-17)
The v-Crk protein was originally isolated as the oncogene fusion product of the CT10 chicken retrovirus. Cellular homologues of v-Crk include Crk, which encodes two alternatively spliced proteins (CrkI and CrkII), and CrkL. Though CrkI/II proteins are elevated in several
Chiyi Xiong et al.
Scientific reports, 10(1), 520-520 (2020-01-18)
The tyrosine kinase receptor EphB4 is frequently overexpressed in ovarian and other solid tumors and is involved in interactions between tumor cells and the tumor microenvironment, contributing to metastasis. Trans-interaction between EphB4 and its membrane-bound ligand ephrin B2 (EFNB2) mediates
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