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Merck
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L4895

Lectin from Triticum vulgaris (wheat)

FITC conjugate, lyophilized powder

Synonym(s):

Wheat germ agglutinin, WGA

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352202

Key Documents

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Product Name

Lectin from Triticum vulgaris (wheat), FITC conjugate, lyophilized powder

conjugate

FITC conjugate

form

lyophilized powder

potency

<50 μg/mL agglutination activity

composition

Protein, ~50% Lowry

extent of labeling

~2 mol FITC per mol protein

storage temp.

2-8°C

Quality Level

200

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Physical form

Contains phosphate buffer salts

Preparation Note

Conjugates are prepared from affinity purified lectin.

Biochem/physiol Actions

WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.
Lectin plays a key role in glycoconjugate characterizing, imaging and targeting. Its use in a microarray assay, enable efficient glycome profiling. In plants and fungi, lectin defends against pathogens/feeders. Lectin also plays a major role in host recognition and tissue adhesion.

General description

Lectins are carbohydrate-binding proteins. They are omnipresent and usually found in fungi, plants and animals. The secondary structure of this protein is rich in β-strands and possesses carbohydrate binding sites on the surface.

Application

For the fluorescent detection of glycoproteins containing β(1→4)-N-acetyl-D-glucosamine
Lectin from Triticum vulgaris (wheat) has been used:
  • to treat transfected cells for immunofluorescence
  • to stereotaxically inject into mice for neuronal tracing
  • in immunohistochemistry for the analysis of myocytes

Packaging

Package size based on protein content.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

动植物源性产品
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Certificates of Analysis (COA)

Lot/Batch Number
0000477765
0000477765
0000477764
0000477764
0000419115

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Yihua Bei et al.
Journal of cellular and molecular medicine, 21(8), 1648-1655 (2017-03-18)
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Science signaling, 12(577) (2019-04-18)
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Circulation (2011)
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Lennington JB, et al.
The Journal of Neuroscience, 31(37), 13078-13087 (2011)
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Product Information Sheet - L4895

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