L4524
Lipopolysaccharides from Escherichia coli O55:B5
purified by ion-exchange chromatography, TLR ligand tested
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Synonym(s):
LPS
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biological source
Escherichia coli (O55:B5)
Quality Level
form
lyophilized powder
purified by
ion-exchange chromatography
impurities
<1% Protein
<1% RNA
color
white to yellow cast
solubility
water: soluble
shipped in
ambient
storage temp.
2-8°C
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General description
This product is extracted from E. coli serotype O55:B5 and purified by ion exchange. The source strain is CDC 1644-70. The LPS O55:B5 has been used to stimulate human peritoneal macrophages at 1 ng/mL and to stimulate equine peritoneal macrophages at 1-100 ng/mL.
Application
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).
Biochem/physiol Actions
Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.
Preparation Note
The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.
Other Notes
To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
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Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 2 Oral
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Yoshitaka Shirasaki et al.
Scientific reports, 4, 4736-4736 (2014-04-23)
Protein secretion, a key intercellular event for transducing cellular signals, is thought to be strictly regulated. However, secretion dynamics at the single-cell level have not yet been clarified because intercellular heterogeneity results in an averaging response from the bulk cell
Hung-Yen Chou et al.
PloS one, 13(5), e0196890-e0196890 (2018-05-09)
The purple sea urchin, Strongylocentrotus purpuratus, has a complex and robust immune system that is mediated by a number of multi-gene families including the SpTransformer (SpTrf) gene family (formerly Sp185/333). In response to immune challenge from bacteria and various pathogen-associated
Arij Faksh et al.
Pediatric research, 79(3), 391-400 (2015-11-06)
Antenatal inflammation and preterm birth are associated with the development of airway diseases such as wheezing and asthma. Utilizing a newborn mouse model, we assessed the effects of maternal inflammation and postnatal hyperoxia on the neonatal airway. Pregnant C57/Bl6 dams
Laura B Fanning et al.
PloS one, 8(2), e57007-e57007 (2013-02-23)
Leukocyte Immunoglobulin-like Receptor B4 (LILRB4) null mice have an exacerbated T helper cell type 2 (Th2) immune response and pulmonary inflammation compared with Lilrb4(+/+) animals when sensitized intranasally with ovalbumin (OVA) and low-dose lipopolysaccharide (LPS) followed by challenge with OVA.
Up-regulated TLR4 in cardiomyocytes exacerbates heart failure after long-term myocardial infarction.
Li Liu et al.
Journal of cellular and molecular medicine, 19(12), 2728-2740 (2015-08-21)
It remains unclear whether and how cardiomyocytes contribute to the inflammation in chronic heart failure (CHF). We recently reviewed the capacity of cardiomyocytes to initiate inflammation, by means of expressing certain immune receptors such as toll-like receptors (TLRs) that respond
Articles
Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.
Related Content
Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.
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