L3289
Lysis solution for blood
sufficient for 100 reactions, for molecular biology
Synonym(s):
Blood direct PCR, Blood lysis buffer, Extract-N-Amp blood direct PCR lysis solution
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About This Item
grade
for molecular biology
Quality Level
form
liquid
usage
sufficient for 100 reactions
storage temp.
−20°C
Related Categories
General description
Lysis solution for blood in combination with neutralization solution for blood (N9784) aids in rapid extraction and neutralization of DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.
This lysis solution is a component of the Extract-N-Amp™ and the REDExtract-N-Amp™ Blood PCR Kits
This lysis solution is a component of the Extract-N-Amp™ and the REDExtract-N-Amp™ Blood PCR Kits
Application
Lysis solution for blood has been used for lysis of blood cells for DNA extraction prior to polymerase chain reaction (PCR) amplification. It has also been used for the isolation of genomic DNA for CRISPR-Cas9 gene editing.
Legal Information
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
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Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Identification by random mutagenesis of functional domains in KREPB5 that differentially affect RNA editing between life cycle stages of Trypanosoma brucei
Molecular and Cellular Biology, MCB-00790 (2015)
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Mutations in the Leucine rich repeat kinase 2 (LRRK2) gene are found in both familial and sporadic Parkinson's disease (PD), and are also associated with immune-related disorders including Crohn's disease (CD) and leprosy. We have generated two homozygous LRRK2 knockout
Haematologica, 99(1), 155-162 (2013-07-23)
The B-cell compartment in which multiple myeloma stem cells reside remains unclear. We investigated the potential presence of mature, surface-membrane immunoglobulin-positive B lymphocytes clonally related to the tumor bone marrow plasma cells among different subsets of peripheral blood B cells
Identification by random mutagenesis of functional domains in KREPB5 that differentially affect RNA editing between life cycle stages of Trypanosoma brucei
Molecular and cellular biology, MCB-00790 (2015)
Stem cell research, 41, 101602-101602 (2019-11-08)
Loss of function mutations in PARK2 (encoding PARKIN) cause autosomal recessive Parkinson's disease (PD), which often manifests at a juvenile age. Molecular and biochemical studies show that PARKIN functions as an E3 ubiquitin ligase controlling mitochondrial homeostasis. Yet, the exact
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