L3160
Monoclonal Anti-Leptin antibody produced in mouse
clone LEP-13, ascites fluid
Sign Into View Organizational & Contract Pricing
All Photos(1)
Anti-OB
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
LEP-13, monoclonal
mol wt
antigen 16 kDa
contains
15 mM sodium azide
species reactivity
human
technique(s)
indirect ELISA: suitable
radioimmunoassay: suitable
western blot: 1:1,000 using recombinant human leptin
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... LEP(3952)
General description
Leptin is a hormone secreted by adipocytes and regulates energy homeostasis, inflammation and neuroendocrine processes, synaptic formation and memory condensation. The physiological effects of leptin are mediated by leptin receptors, ObR and LepR. The receptors are present in brain, lungs, kidneys, liver and adipose tissue. Leptin is the key regulator of obesity and acts directly on neuronal networks to regulate feeding, metabolism, gastric mobility and energy homeostasis. The pathways that mediate the effect of leptin are JAK/STAT, PI3K and JNK. Deficiency of leptin may be a congenital condition and results in thyroid axis abnormalities.. Monoclonal Anti-Leptin reacts specifically with human leptin. A weak cross reactivity with mouse leptin is observed in radioimmunoassay.
Monoclonal Anti-Leptin (OB) (mouse IgG isotype) is derived from the LEP-13 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant human leptin. Leptin (also known as OB, the product of the ob gene) is a 16 kDa, 146 amino acid residue, non-glycosylated polypeptide, secreted by the mature adipocyte. It is strongly expressed in white adipose tissue, and is absent or expressed at extremely low levels in other tissues. The human leptin molecule is translated as a 167 amino acid residue polypeptide, with the first 21 amino acid residues cleaved as a signal peptide.
Specificity
The antibody reacts specifically with human leptin. By RIA, weak cross-reactivity has been observed with recombinant mouse leptin.
Immunogen
recombinant human leptin.
Application
Monoclonal Anti-Leptin antibody produced in mouse has been used in western blotting. It may be used in radioimmunoassay (RIA) and enzyme linked immunosorbent assay (ELISA).
Biochem/physiol Actions
Leptin is hypothesized to be a ′′satiety factor′′, because the absence of this factor was associated with hyperphagia and obesity. Leptin is involved in appetite regulation. Human obesity is often associated with increased blood leptin levels. Leptin acts through discrete receptors and distant targets to create a feedback loop for body weight regulation. With respect to the target of leptin action, the hypothalamus has received a great deal of attention. The leptin system has also been proposed to contribute to early hematopoiesis.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Superactive human leptin antagonist reverses leptin-induced excessive progesterone and testosterone secretion in porcine ovarian follicles by blocking leptin receptors
Journal of Physiology And Pharmacology, 66(3), 39-46 (2015)
Bisphenol A induces leptin receptor expression, creating more binding sites for leptin, and activates the JAK/Stat, MAPK/ERK and PI3K/Akt signalling pathways in human ovarian cancer cell
Toxicology Letters, 210(3), 332-337 (2012)
Narrative review: the role of leptin in human physiology: emerging clinical applications
Annals of Internal Medicine, 152(2), 93-93 (2010)
Annals of the New York Academy of Sciences, 1264, 64-71 (2012-04-26)
Leptin, an adipocyte-derived cytokine, crosses the blood-brain barrier to act on many regions of the central nervous system (CNS). It participates in the regulation of energy balance, inflammatory processes, immune regulation, synaptic formation, memory condensation, and neurotrophic activities. This review
Yonsei medical journal, 53(4), 671-679 (2012-06-06)
Leptin, a peptide hormone secreted by adipocytes in proportion of the amount of energy stored in fat, plays a central role in regulating human energy homeostasis. In addition, leptin plays a significant permissive role in the physiological regulation of several
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service