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Merck
CN

L3001

Lipase from wheat germ

Type I, lyophilized powder, 5-15 units/mg solid

Synonym(s):

Triacylglycerol acylhydrolase, Triacylglycerol lipase

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About This Item

CAS Number:
9001-62-1
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-619-9
EC Number:
3.1.1.3 (BRENDA, IUBMB)
MDL number:
MFCD00131509
Specific activity:
5-15 units/mg solid
Biological source:
wheat (germ)

Key Documents

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InChI key

QWZUIMCIEOCSJF-CHHCPSLASA-N

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;

biological source

wheat (germ)

type

Type I

form

lyophilized powder

specific activity

5-15 units/mg solid

UniProt accession no.

Q8L6B0

storage temp.

2-8°C

Quality Level

200

Gene Information

wheat ... lipase(543006)

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General description

Lipases belong to the class of hydrolases. Microbes of plant and animal basis mainly produce lipase enzyme. It can also be produced by marine organisms.

Application

Lipase from wheat germ has been used:
  • to prepare enzyme solution for the biodegradability test
  • to treat diacetates of Mep-Xylp, to generate monoacetylated xylosides
  • to test its efficacy in selenium speciation analysis and to determine the contribution that impurities could make to Se determination in real samples

Biochem/physiol Actions

Lipases are used in detergent, food, leather, pharmaceutical and dairy industries. It has several characteristics like substrate specificity, stereospecificity and regioselectivity. It also participates in various bioconversion reactions like, hydrolysis, esterification, acidolysis and aminolysis.
Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Preparation Note

Prepared essentially by method of Singer, T.P., J. Biol. Chem., 174, 11 (1948).

Analysis Note

Contains acid phosphatase.

Other Notes

One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.4 at 37 °C. (This is equivalent to approx. 10 microliters of CO2 in 30 minutes.)

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

低风险生物材料
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Certificates of Analysis (COA)

Lot/Batch Number
0000522191
0000522191
0000512074
0000512074
0000425592

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Ondřej Dlouhý et al.
Cells, 10(9) (2021-09-29)
Build-up of the energized state of thylakoid membranes and the synthesis of ATP are warranted by organizing their bulk lipids into a bilayer. However, the major lipid species of these membranes, monogalactosyldiacylglycerol, is a non-bilayer lipid. It has also been
Marine fungal and bacterial isolates for lipase production: A comparative study
Advances in Food and Nutrition Research, 78, 71-94 (2016)
Preparation and characterization of poly (ethylene glycol)-crosslinked reacetylated chitosans
Dal P A, et al.
Carbohydrate Polymers, 42(2), 201-206 (2000)
Cassilda Cunha-Reis et al.
Journal of tissue engineering and regenerative medicine, 7(1), 39-50 (2011-11-30)
The establishment of non-invasive analytical tools for assessing the in-situ use of biomaterials for surgical implants or scaffolds in tissue engineering and polymer-based therapies is fundamental. This study established a method for fluorescent tracking of the degradation of a chitosan
Substrate specificity of acetylxylan esterase from Schizophyllum commune: mode of action on acetylated carbohydrates
Biely P, et al.
Biochimica et Biophysica Acta, 1298(2), 209-222 (1996)
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