L2650
Lectin from Glycine max (soybean)
peroxidase conjugate, lyophilized powder
Synonym(s):
SBA, Soybean agglutinin
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
biological source
Glycine max (soybean)
conjugate
peroxidase conjugate
Assay
85.00-100.00% (Warburg-Christian)
form
lyophilized powder
potency
<80 μg per mL agglutination activity
peroxidase activity
25-100 units/mg protein
composition
Protein, ~95% modified Warburg-Christian
technique(s)
western blot: suitable
UniProt accession no.
storage temp.
−20°C
Gene Information
soybean ... LE1(100818710)
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Application
General Western Blot Protocol:
- Glycoprotein sample size: 500ng
- Lectin Concentration: 0.1ug/ml
- Load samples at 500 ng of glycoprotein per lane
- Run 4-20% Bis-Tris SDS page gel
- Transfer gel to a PVDF membrane
- Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
- Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
- Wash membrane 5 x 5 minutes with 25ml RIPA buffer
- Detect using chemiluminescent substrate (CPS1-120)
Biochem/physiol Actions
SBA is not blood group specific but has an affinity for N-acetyl-D-galactosamine.
Unit Definition
One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0, at 20 °C.
Physical form
Contains sodium citrate
Preparation Note
Conjugates are prepared from affinity purified lectin.
Prepared from peroxidase type VI (P8375) using a modification of the method of O′Sullivan, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.
Analysis Note
Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hr incubation at 25 °C.
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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