Lectin from Ricinus communis (castor bean) Toxin RCA₆₀–Peroxidase labeled

Ricinus communis agglutinin (RCA) occurs in two forms designated RCA₆₀ and RCA₁₂₀ according to their mol. wt. of approx. 60,000 and 120,000 respectively.¹ Neither RCA₆₀ nor RCA₁₂₀ is blood group specific. RCA₆₀, also referred to as RCA_II,² Ricin D³ or RCL III⁴ is extremely toxic, inhibits protein synthesis and has an affinity for N-acetyl-D-galactosamine. RCA₁₂₀, also referred to as RCA_I² or RCL (I + II)⁴ is an agglutinin and has an affinity for terminal β-D-galactosyl residues.¹
Conjugates are prepared from affinity purified toxin, RCA₆₀ or agglutinin RCA₁₂₀.

Package size based on protein content

One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20°C.

Lyophilized powder containing sodium citrate

Prepared from peroxidase type VI using two step glutaraldehyde method of Avrameas, S. and Ternyck, T., Immunochemistry, 8, 1175 (1971).
Repurified after conjugation by affinity chromatography.

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 7.2, of a 1 mg protein per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hour incubation at 25°C.