L2507
Lectin from Arachis hypogaea (peanut)
agarose conjugate, saline suspension
Synonym(s):
PNA, Peanut agglutinin
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
conjugate
agarose conjugate
form
saline suspension
extent of labeling
2-4 mg per mL
matrix
cross-linked 4% beaded agarose
matrix activation
cyanogen bromide
matrix attachment
amino
matrix spacer
1 atom
capacity
~1.5 mg/mL binding capacity (asialofetuin)
storage temp.
2-8°C
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Application
Used for the purification of O-linked glycoproteins.
Biochem/physiol Actions
PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase treated erythrocytes. PNA has potent anti-T activity similar to the anti-T antibody in human sera. The lectin can be used to distinguish between human lymphocyte subsets.
Physical form
Suspension in 1.0 M NaCl containing 15 Mm Sodium azide.
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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N Shimoda et al.
Journal of biochemistry, 102(3), 657-664 (1987-09-01)
Receptors for peanut agglutinin (PNA) were isolated from Kato III human gastric cancer cells by affinity chromatography on PNA agarose, and were labeled by the galactose oxidase-NaB3H4 method. Alkaline NaBH4 treatment of the labeled receptors released two small oligosaccharide alcohols
Jie Fan et al.
Investigative ophthalmology & visual science, 49(6), 2384-2389 (2008-02-26)
The Rpe65-/- mouse, used as a model for Leber congenital amaurosis, has slow rod degeneration and rapid cone loss, presumably because of the mistrafficking of cone opsins. This animal does not generate 11-cis retinal, and both cone loss and rod
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