L2380
Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)
lyophilized powder
Synonym(s):
Bandeirea simplicifolia agglutinin, BS-I
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder
form
lyophilized powder
potency
<20 μg per mL agglutination activity (using human blood group B erythrocytes)
composition
Protein, ≥90% biuret
impurities
salt, free
storage temp.
2-8°C
Quality Level
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Biochem/physiol Actions
BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.
Preparation Note
Purified by affinity chromatography using the method of Hayes, C. E., and Goldstein, I. J., J. Biol. Chem., 249, 1904 (1974).
Analysis Note
Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.
Other Notes
BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A4 has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
低风险生物材料
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