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L1795

Sigma-Aldrich

Anti-PLAGL1 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-LOT-1, Anti-Lost on transformation 1, Anti-PLAL1, Anti-Pleiomorphic adenoma-like protein 1, Anti-Tumor supressor ZAC

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~50 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

indirect immunofluorescence: 0.5-1 μg/mL using paraformaldehyde fixed HEK-293T cells over expressing human PLAGL1
western blot: 1-2 μg/mL using lysates of HEK-293T cells over expressing human PLAGL1

UniProt accession no.

Q9UM63

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PLAGL1(5325)

General description

The gene PLAGL1 (pleiomorphic adenoma-like protein 1), PLAGL2 and PLAG1 form a subfamily of PLAG family of zinc finger proteins that recognize DNA and RNA. The three members share similarity at the amino-terminal, but are functionally different, varying in their DNA binding capacities. The gene is mapped to human chromosome 6q24.2, a region implicated in several cancers.

Specificity

Anti-PLAGL1 (C-terminal) specifically recognizes human PLAGL1.

Application

Anti-PLAGL1 (C-terminal) antibody produced in rabbit may be used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

PLAGL1 gene expression is downregulated in a methylation-sensitive manner. PLAGL1 is an imprinted gene that mediates an imprinted gene network that plays an important role in embryonic growth control.
The maternally-imprinted gene, PLAGL1 (pleiomorphic adenoma-like protein 1), functions as an oncogene as well as a tumor suppressor depending on the cell context. It has been found to exhibit anti-proliferative properties and regulate apoptosis and cell-cycle arrest via p53.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Certificates of Analysis (COA)

Lot/Batch Number
079K4767

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Zac1 regulates an imprinted gene network critically involved in the control of embryonic growth
Varrault A, et al.
Developmental Cell, 11(5), 711-722 (2006)
Transcriptional activation capacity of the novel PLAG family of zinc finger proteins.
Kas K
The Journal of Biological Chemistry, 273, 23026-23032 (1998)
The tumorigenic diversity of the three PLAG family members is associated with different DNA binding capacities.
Hensen K
Cancer Research, 62, 1510-1517 (2002)
Anne-Lise Peille et al.
PloS one, 8(11), e80741-e80741 (2013-11-22)
Soft tissue sarcomas (STS) are rare, complex tumors with a poor prognosis. The identification of new prognostic biomarkers is needed to improve patient management. Our aim was to determine the methylation status of the 118 CpG sites in the PLAGL1

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