L1378
L-Lactic Dehydrogenase from bovine muscle
Type X, ammonium sulfate suspension, ≥600 units/mg protein
Synonym(s):
(S)-Lactate: NAD+ oxidoreductase, L-LDH, LAD, LD, Lactate
biological source
bovine muscle
Quality Level
type
Type X
Assay
3.0—25.0 mg protein/mL (biuret)
form
ammonium sulfate suspension
specific activity
≥600 units/mg protein
manufacturer/tradename
Sigma-Aldrich
technique(s)
activity assay: suitable
color
white
pH
7.5
application(s)
life science and biopharma
foreign activity
pyruvate kinase ≤0.01%
storage temp.
2-8°C
Gene Information
cow ... LDHA(281274), LDHB(281275)
General description
Research Area: Cell Signaling
Lactate dehydrogenase (LDH) is a key enzyme in the anaerobic metabolic pathway and falls under the class of oxidoreductases. This enzyme is found in a wide range of organisms, including both plants and animals. It is widely distributed across all tissues. LDH is primarily a cytoplasmic enzyme, with particularly high concentrations in muscle, liver, and kidney tissues.
Lactate dehydrogenase (LDH) is a key enzyme in the anaerobic metabolic pathway and falls under the class of oxidoreductases. This enzyme is found in a wide range of organisms, including both plants and animals. It is widely distributed across all tissues. LDH is primarily a cytoplasmic enzyme, with particularly high concentrations in muscle, liver, and kidney tissues.
Application
L-Lactic Dehydrogenase from bovine muscle has been used in lactate assay to assess lactate tissue accumulation.
Biochem/physiol Actions
Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.
Lactate dehydrogenase (LDH) plays a critical role as a checkpoint in gluconeogenesis and DNA metabolism. It plays a vital role in maintaining homeostasis during periods of low oxygen availability. As an oxidoreductase enzyme, lactate dehydrogenase catalyzes the reversible conversion of pyruvate to lactate using NADH. This enzyme is crucial for the anaerobic metabolism of glucose when oxygen is either absent or in short supply. LDH is the sole serum biomarker effective for evaluating metastatic melanomas.
Physical form
Crystalline suspension in 2.4 M (NH4)2SO4 solution, pH 6.0
Analysis Note
Protein determined by biuret.
Other Notes
One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
低风险生物材料
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Metabolic recovery from submaximal exercise in hypoxia acclimated high altitude deer mice (Peromyscus maniculatus)
Dessureault LM, et al.
Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, 274, 111004-111004 (2024)
Biochemistry, lactate dehydrogenase
Farhana A and Lappin SL
StatPearls [Internet] (2020)
Deshdeepak Sahni et al.
Journal of neurosurgery. Pediatrics, 11(5), 575-583 (2013-03-12)
Graphene possesses unique electrical, physical, and chemical properties that may offer significant potential as a bioscaffold for neuronal regeneration after spinal cord injury. The purpose of this investigation was to establish the in vitro biocompatibility of pristine graphene for interface
Bécaye Fall et al.
Malaria journal, 12, 107-107 (2013-03-21)
In 2006, the Senegalese National Malaria Control Programme recommended artemisinin-based combination therapy (ACT) as the first-line treatment for uncomplicated malaria. Since the introduction of ACT, there have been very few reports on the level of resistance of P. falciparum to
D Steffens et al.
Journal of biomedical nanotechnology, 9(4), 710-718 (2013-04-30)
The association of stem cells (SCs) with biomaterials promises to be the protagonist for future regenerative medicine in the treatment of tissue and organ lesions. Stem cells were cultivated in scaffolds constructed by the electrospinning technique, using poly-D,L-lactic acid (PDLLA)
Articles
Instructions for working with enzymes supplied as ammonium sulfate suspensions
以硫酸铵悬浮液形式提供的酶的使用指南
Related Content
QC Methods
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service