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L1254

Sigma-Aldrich

L-Lactic Dehydrogenase from rabbit muscle

Type XI, lyophilized powder, 600-1,200 units/mg protein

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Synonym(s):
Anaerobic Lactate Dehydrogenase, Lactate, NAD-lactate dehydrogenase, (S)-Lactate: NAD+ oxidoreductase, L-LDH, LAD, LD
CAS Number:
9001-60-9
Enzyme Commission number:
1.1.1.27 (BRENDA, IUBMB)
EC Number:
232-617-8
MDL number:
MFCD00131466
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

rabbit muscle

Quality Level

200

type

Type XI

form

lyophilized powder

specific activity

600-1,200 units/mg protein

mol wt

140 kDa

composition

protein, 90-100%

storage condition

(Keep container tightly closed in a dry and well-ventilated place)

technique(s)

activity assay: suitable

color

white

foreign activity

pyruvate kinase, myokinase, malic dehydrogenase, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase and α-glycerophosphate dehydrogenase ≤0.01%

storage temp.

−20°C

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General description

Research area: Cell Signaling
Lactic Dehydrogenase (LDH) has a total molecular weight of 140 kDa and is composed of 4 subunits which are designated M subunit (muscle) and H subunit (heart). These subunits may be mixed in any of 5 combinations (M4, M3H1, M2H2, MH3, and H4). Skeletal muscle contains LDH that is predominately M4 with some small amounts of M3H and traces of H2H2. The H and M subunits are quite similar in molecular weight, but differ substantially in amino acid composition. Rabbit muscle LDH dissociates into dimeric species (MW = ~70 kDa) in acetate-chloride at pH 5.0, the dissociation is reversible. Biochemistry, 13, 3527-3531 (1974). Oxidizes glyoxylate and lactate.
Isoelectric point: 8.4-8.6
Optimal pH : 7.5 .

Application

L-Lactic Dehydrogenase from rabbit muscle has been used:
  • as a component of activation and relaxing solution in ATPase activity and isometric steady-state tension measurements with muscle fiber
  • in Trypanosoma congolense pyruvate kinase activity assay
  • in pyruvate kinase (PK) assay with rice plastidic PK enzyme OsPK2

Biochem/physiol Actions

Muscle-type Lactic Dehydrogenase (LDH) participates in metabolic pathways and its activity is essential for anaerobic glycolysis. LDH activity is inhibited by ascorbate. LDH regenerates nicotinamide adenine dinucleotide (NAD+) from NADH and is industrially useful in poly(lactic acid) production. In the absence of oxygen, LDH participates in a fermentation reaction, catalyzes pyruvate into lactic acid, and oxidizes nicotinamide adenine dinucleotide (NADH) to NAD+. Therefore, LDH mediates the production of NAD+ essential anaerobic glycolysis pathway. Through this LDH helps maintain the physiological and biochemical functions of the cell in the absence of oxygen.
Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

Unit Definition

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

Analysis Note

Protein determined by biuret.

antibody

Product No.
Description
Pricing

enzyme

Product No.
Description
Pricing

C3755

Creatine Phosphokinase from rabbit muscle, Type I, salt-free, lyophilized powder, ≥150 units/mg protein

L1254

L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein

L1378

L-Lactic Dehydrogenase from bovine muscle, Type X, ammonium sulfate suspension, ≥600 units/mg protein

G8255

Glutamic-Pyruvic Transaminase from porcine heart, lyophilized powder, ≥75 units/mg protein

L9636

D-Lactic Dehydrogenase from Staphylococcus epidermidis, lyophilized powder, ≥80 units/mg solid

L7525

L-Lactic Dehydrogenase from porcine heart, ammonium sulfate suspension, ≥200 units/mg protein

M3003

Myokinase from rabbit muscle, ammonium sulfate suspension, 1,500-3,000 units/mg protein (biuret)

G6751

α-Glycerophosphate Dehydrogenase from rabbit muscle, Type I, ammonium sulfate suspension, 100-300 units/mg protein

P3397

Phosphoglucomutase from rabbit muscle, ammonium sulfate suspension, ≥100 units/mg protein

P7768

Pyruvate Kinase from rabbit muscle, Type VII, buffered aqueous glycerol solution, 350-600 units/mg protein

G2267

Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle, lyophilized powder, ≥75 units/mg protein

P9136

Pyruvate Kinase from rabbit muscle, Type III, lyophilized powder, 350-600 units/mg protein

A2714

Aldolase from rabbit muscle, lyophilized powder, ≥8.0 units/mg protein

L2625

L-Lactic Dehydrogenase from bovine heart, Type III, ammonium sulfate suspension, ≥500 units/mg protein

T6258

Triosephosphate Isomerase from rabbit muscle, Type X, lyophilized powder, ≥3,500 units/mg protein

T2391

Triosephosphate Isomerase from rabbit muscle, Type III-S, ammonium sulfate suspension, ≥4,000 units/mg protein

P0294

Pyruvate Kinase/Lactic Dehydrogenase enzymes from rabbit muscle, For the Determination of ADP, buffered aqueous glycerol solution

59747

Lactic Dehydrogenase, recombinant from E. coli, ≥90 U/mg

SAE0049

L-Lactate Dehydrogenase (LDHA), from human, recombinant, expressed in E. coli, aqueous solution

inhibitor

Product No.
Description
Pricing

related product

Product No.
Description
Pricing

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

动植物源性产品

Certificates of Analysis (COA)

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Matthew Warren Eggert et al.
Applied biochemistry and biotechnology, 165(2), 676-686 (2011-06-01)
In order to evaluate the effectiveness of L: -lactate dehydrogenase (LDH) from rabbit muscle as a regenerative catalyst of the biologically important cofactor nicotinamide adenine dinucleotide (NAD), the kinetics over broad concentrations were studied to develop a suitable kinetic rate
Farhana A and Lappin. SL
Biochemistry (2022)
Belén Torrado et al.
Biomedical optics express, 12(7), 3760-3774 (2021-08-31)
We describe a method based on a pair of transmission filters placed in the emission path of a microscope to resolve the emission wavelength of every point in an image. The method can be applied to any type of imaging
John Jeshurun Michael et al.
Journal of the American Heart Association, 5(3), e002777-e002777 (2016-03-24)
We hypothesized that the functional effects of R206L-a rat analog of the dilated cardiomyopathy (DCM) mutation R205L in human cardiac troponin T (TnT)-were differently modulated by myosin heavy chain (MHC) isoforms and T204E, a protein kinase C (PKC) phosphomimic of
Yicong Cai et al.
Plant biotechnology journal, 16(11), 1878-1891 (2018-03-27)
Starch is the main form of energy storage in higher plants. Although several enzymes and regulators of starch biosynthesis have been defined, the complete molecular machinery remains largely unknown. Screening for irregularities in endosperm formation in rice represents valuable prospect
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For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein

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