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L0777

Sigma-Aldrich

Lipase from Aspergillus oryzae

solution, ≥100,000 U/g, white, beige

Synonym(s):

AOL, Lipolase 100L

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About This Item

CAS Number:
9001-62-1
Enzyme Commission number:
3.1.1.3.
EC Number:
232-619-9
MDL number:
MFCD00131509
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Aspergillus sp. (Aspergillus oryzae)

Quality Level

200

form

solution

specific activity

≥100,000 U/g

storage condition

(Tightly closed. Dry)

technique(s)

cell based assay: suitable

color

beige
 white

UniProt accession no.

I8ABR3
I8ADK3

storage temp.

2-8°C

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;

InChI key

QWZUIMCIEOCSJF-CHHCPSLASA-N

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General description

Research area: Cell Signaling

Lipase is derived from Aspergillus oryzae by ammonium sulfate precipitation and chromatography.Lipases or triacylglycerol acyl hydrolases are a group of hydrolase enzymes that are usually found in humans and animals with monogastric stomachs. Lipase enzymes are generally formed in the pancreas and stomach where they aid in fat and lipids digestion.(2)

Application

Lipase from Aspergillus oryzae has been used:
  • as a control enzyme in activity assays
  • as a component in lipolase stock solution for the hydrolysis experiments
  • to incubate scaffolds for lipase-accelerated degradation experiments
  • as the lipolytic enzyme standard to detect lipolytic enzymatic activity via chromogenic agar plates and zymography

Biochem/physiol Actions

Lipase is widely used in several industries including food and pharmaceuticals. It mediates the hydrolysis of fats and oil. Lipase is utilized for peptide synthesis and in the detergent industry. It is active in the range of pH 2-5 and temperature between 30-50°C. Metal ions such as Fe2+, Fe3+, and Cu2+ prevent the action of lipase. A less polar organic solvent is preferred for high stability.Lipases play a crucial role in digestion as well as the transportation and processing of dietary lipid substrates by catalyzing the hydrolysis of ester bonds in lipid substrates. The lipase from Aspergillus oryzae (AOL), a multipurpose biocatalyst can be used in the kinetic resolution of a biotin intermediate lactone,α-lipoic acid, and 1-phenylethanol. Additionally, it has the ability to stereoselectively catalyze the hydrolysis of ethyl 2-(4-hydroxyphenoxy) propanoate and its analogs, which are key intermediates in the production of aryloxyphenoxypropionate herbicides. AOL serves as a promising biocatalyst in the esterification of a series of short-chain acids and alcohols to produce flavor esters. It can be used to catalyze the esterification of lauric acid with aromatic alcohol-benzyl alcohol.

Preparation Note

Produced by submerged fermentation of a genetically modified Aspergillus oryzae microorganism

Legal Information

A product of Novozyme Corp.

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L9031

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L9656

Lipoprotein Lipase from Burkholderia sp., lyophilized powder, ≥50,000 units/mg solid

L4277

Lipase from Aspergillus oryzae, ≥20,000 U/g

62335

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62287

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62288

Lipase B Candida antarctica, recombinant from Aspergillus oryzae, powder, beige, ~9 U/mg

62298

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62301

Lipase from Aspergillus niger, powder (fine), ~200 U/g

62302

Lipase from Candida rugosa, lyophilized, powder (fine), 15-25 U/mg

62305

Lipase from Rhizopus oryzae, powder (fine), ~10 U/mg

62309

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80612

Lipase from Rhizopus oryzae, powder, light brown, ≥30 U/mg

62310

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73940

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534781

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Amano Lipase M from Mucor javanicus, ≥10,000 U/g, pH 7.0, 40 °C (Optimum pH and temperature)

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437707

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substrate

Product No.
Description
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Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the buffer composition which Product L0777, Lipase is offered in?

    Product L0777, Lipase is offered as an enzyme solution containing 73% (w/v) water, 25% (w/v) propylene glycol, 2% (w/v) lipase, and 0.5% calcium chloride.

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E E van Haaften et al.
Acta biomaterialia, 92, 48-59 (2019-05-21)
To maintain functionality during in situ vascular regeneration, the rate of implant degradation should be closely balanced by neo-tissue formation. It is unknown, however, how the implant's functionality is affected by the degradation of the polymers it is composed of.
Microbial Enzyme in Food Biotechnology
Singh P and Kumar S
Enzymes in Food Biotechnology, 19-28 (2019)
E E van Haaften et al.
Acta biomaterialia, 92, 48-59 (2019-05-21)
To maintain functionality during in situ vascular regeneration, the rate of implant degradation should be closely balanced by neo-tissue formation. It is unknown, however, how the implant's functionality is affected by the degradation of the polymers it is composed of.
Hong De Yan et al.
3 Biotech, 9(7), 265-265 (2019-06-21)
The lipase from Aspergillus oryzae was modified with a surfactant and then observed to exhibit high catalytic efficiency and enantioselectivity for the kinetic resolution of (RS)-1-phenylethanol. The influential factors of the modified-lipase preparation were investigated, including the surfactant source, the
Andre Mong Jie Ng et al.
Molecules (Basel, Switzerland), 26(6) (2021-04-04)
Lipases and esterases are important catalysts with wide varieties of industrial applications. Although many methods have been established for detecting their activities, a simple and sensitive approach for picogram detection of lipolytic enzyme quantity is still highly desirable. Here we
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