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Safety Information

L032000

Sigma-Aldrich

DCI Activator

0.25 M

Synonym(s):

DCI activator solution

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About This Item

UNSPSC Code:
12352200

type

for DNA synthesis
for DNA synthesis

product line

Proligo Reagents

storage condition

dry at room temperature

concentration

29-32 g/L (DCI content, titration)

impurities

≤30 ppm water content (Karl Fischer)
≤30 ppm water content (Karl Fischer)

color

clear
clear

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Preparation Note

Dicyanoimidazole / Acetonitrile 3/100 (w/v)

Other Notes

Activator solution, containing 4,5-dicyanoimidazole (DCI) in acetonitrile, are mixed with solutions of phosphoramidites during the extension step.
The activator reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the detritylated 5′hydroxyl group of the growing oligonucleotide chain.

Regulatory Information

新产品

Certificates of Analysis (COA)

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