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Safety Information

KEM0020

Sigma-Aldrich

T4 DNA Ligase

Ultra-pure enzyme for nucleic acid modifications

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Select a Size

4 X 25 ML
CN¥940.97
100 ML
CN¥1,006.36
800 ML
CN¥2,710.17

CN¥940.97

Available to ship onApril 25, 2025Details

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4 X 25 ML
CN¥940.97
100 ML
CN¥1,006.36
800 ML
CN¥2,710.17

About This Item

CN¥940.97

Available to ship onApril 25, 2025Details

grade

Molecular Biology

Assay

>99% (SDS-PAGE)

form

buffered aqueous solution

specific activity

300,000 U/mg

concentration

120,000 U/mL

shipped in

dry ice

storage temp.

−20°C

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羟基甲基聚苯乙烯 200-500 mesh, extent of labeling: ~1.1 mmol/g loading

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reaction suitability

reaction type: Fmoc solid-phase peptide synthesis

reaction suitability

reaction type: Fmoc solid-phase peptide synthesis

reaction suitability

reaction type: Fmoc solid-phase peptide synthesis

reaction suitability

reaction type: Boc solid-phase peptide synthesis, reactivity: carboxyl reactive

functional group

alcohol

functional group

alcohol

functional group

alcohol

functional group

alcohol

Quality Level

100

Quality Level

100

Quality Level

-

Quality Level

-

application(s)

peptide synthesis

application(s)

-

application(s)

-

application(s)

-

form

solid

form

-

form

-

form

solid

extent of labeling

1.1 mmol/g loading

extent of labeling

1.0-1.5 mmol/g OH loading

extent of labeling

1.5-2.0 mmol/g OH loading

extent of labeling

~1.1 mmol/g loading

General description

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.

Application

Suitable for:
  • Restriction cloning
  • TA cloning
  • Adapter ligation

Features and Benefits

  • Ultra-purification process for ultimate enzyme performance
  • Highest quality specifications for ultimate product consistency
  • Undetectable DNA and nuclease contamination

Components

Supplied with:KEM0049B (10X T4 DNA Ligase Buffer)

Unit Definition

1 unit is defined as the amount of T4 DNA Ligase required to join 50% of 100 ng of DNA fragments with cohesive termini in 50 μL 1X T4 DNA Ligase Buffer following a 30 minute incubation at 23° C.

Physical form

Supplied in 10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA and 50% glycerol at pH 7.5 @ 25° C.

Other Notes

Source of protein: A recombinant E. coli strain carrying the cloned T4 DNA Ligase gene.
Unit size: 150,000 U

Storage Class Code

10 - Combustible liquids

Regulatory Information

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    Certificates of Analysis (COA)

    Lot/Batch Number
    SLBM9485V
    SLBL8010V
    SLBK1069V
    SLBJ0305V
    SLBH2793V

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    Laure Rittié et al.
    Journal of cell communication and signaling, 2(1-2), 25-45 (2008-09-04)
    Since molecular cloning has become routine laboratory technique, manufacturers offer countless sources of enzymes to generate and manipulate nucleic acids. Thus, selecting the appropriate enzyme for a specific task may seem difficult to the novice. This review aims at providing

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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