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Safety Information

KEM0019

Sigma-Aldrich

T4 DNA Ligase (Rapid)

Ultra-pure enzyme for nucleic acid modifications

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50 EA
¥5,314.03

¥5,314.03

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50 EA
¥5,314.03

About This Item

¥5,314.03

Please contact Customer Service for Availability

grade

Molecular Biology

Assay

>99% (SDS-PAGE)

form

buffered aqueous solution

specific activity

300,000 U/mg

concentration

600,000 U/mL

shipped in

dry ice

storage temp.

−20°C

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MABE222

Anti-JMJD2A Antibody, clone 8B7.1, rabbit monoclonal

Anti-Lysine-specific demethylase 7 Antibody from rabbit, purified by affinity chromatography

ABE498

Anti-Lysine-specific demethylase 7 Antibody

antibody form

affinity isolated antibody

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

biological source

rabbit

biological source

mouse

biological source

rabbit

biological source

rabbit

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

UniProt accession no.

Q9BY66

UniProt accession no.

Q9BY66

UniProt accession no.

O75164

UniProt accession no.

Q6ZMT4

species reactivity

mouse, human

species reactivity

human

species reactivity

human

species reactivity

human

General description

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.

Application

Suitable for:
  • Restriction cloning
  • TA cloning
  • Adapter ligation
  • NGS library construction
  • Other applications requiring high efficiency ligation

Features and Benefits

  • Ultra-purification process for ultimate enzyme performance
  • Highest quality specifications for ultimate product consistency
  • Undetectable DNA and nuclease contamination

Components

Supplied with:KEM0046B (2X Rapid Ligation Buffer)KEM0049B (10X T4 DNA Ligase Buffer)

Unit Definition

1 unit is defined as the amount of DNA Ligase required to join 50% of 100 ng of DNA fragments with cohesive termini in 50 μL 1X DNA Ligase Buffer following a 30 minute incubation at 23° C.

Physical form

Supplied in 10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA and 50% glycerol at pH 7.5 @ 25° C.

Other Notes

Source of protein: A recombinant E. coli strain carrying the T4 DNA Ligase gene.
Unit size: 240,000 U

Storage Class Code

10 - Combustible liquids

Regulatory Information

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Lot/Batch Number
SLBK1070V
SLBJ0306V
SLBH2791V

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Laure Rittié et al.
Journal of cell communication and signaling, 2(1-2), 25-45 (2008-09-04)
Since molecular cloning has become routine laboratory technique, manufacturers offer countless sources of enzymes to generate and manipulate nucleic acids. Thus, selecting the appropriate enzyme for a specific task may seem difficult to the novice. This review aims at providing

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