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Merck
CN

K4377

Anti-Human Kappa Light Chain (Bound and Free) antibody, Mouse monoclonal

clone KP-53, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Human Kappa Light Chain (Bound and Free)

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162517

Key Documents

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Product Name

Anti-Human Kappa Light Chain (Bound and Free) antibody, Mouse monoclonal, clone KP-53, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody product type

secondary antibodies

contains

15 mM sodium azide

technique(s)

indirect ELISA: 1:10,000

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

antibody form

purified from hybridoma cell culture

clone

KP-53

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Application

Monoclonal Anti-Human Kappa Light Chain (Bound and Free) antibody produced in mouse has been used for:
  • immunodetection
  • in western blot analysis
  • in intracellular kappa light chain staining for flow cytometric analysis

Biochem/physiol Actions

The antibody is specific for human kappa (κ) light chains. This antibody is reactive with intact IgG (κ), IgM (κ), free kappa light chains, and Bence-Jones kappa light chains. It is non-reactive with lambda light chains.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-Human Kappa Light Chain (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins G are made up of two identical light chains and two identical heavy chains, that are covalently connected by interchain disulphide bonds. Genes of kappa and lambda constant region are mapped on to human chromosome 2.

Preparation Note

For continuous use, store at 2-8 °C. For extendedstorage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品
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Certificates of Analysis (COA)

Lot/Batch Number
0000480597
0000480597
0000438845
0000438845
0000386532

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Upregulated expression of kappa light chain by Epstein-Barr virus encoded latent membrane protein 1 in nasopharyngeal carcinoma cells via NF-kappaB and AP-1 pathways
Liu H, et al.
Cellular Signalling, 19(2), 419-427 (2007)
Visualisation of intracellular production bottlenecks in suspension-adapted CHO cells producing complex biopharmaceuticals using fluorescence microscopy
Mathias S, et al.
Journal of Biotechnology, 271, 47-55 (2018)
Megan D Lenardon et al.
Cell surface (Amsterdam, Netherlands), 6, 100047-100047 (2020-12-10)
Despite the importance of fungal cell walls as the principle determinant of fungal morphology and the defining element determining fungal interactions with other cells, few scalar models have been developed that reconcile chemical and microscopic attributes of its structure. The
Heterogeneity of aberrant immunoglobulin expression in cancer cells
Hu D, et al.
Cellular & Molecular Immunology, 8(6), 479-479 (2011)
Chloe L Stoyle et al.
The Biochemical journal, 474(18), 3179-3188 (2017-08-09)
Rodent monoclonal antibodies with specificity towards important biological targets are developed for therapeutic use by a process of humanisation. This process involves the creation of molecules, which retain the specificity of the rodent antibody but contain predominantly human coding sequence.
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