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Safety Information

K3144

Sigma-Aldrich

Anti-Keap1 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-Cytosolic inhibitor of Nrf2, Anti-INRF2, Anti-Kelch-like ECH-associated protein 1

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About This Item

UNSPSC Code:
12352203

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~69 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

technique(s)

immunoprecipitation (IP): 5-10 μL using lysates of human Raji cells
western blot: 1:1000-1:2000 using whole extracts of HEK-293T cells expressing human Keap1

UniProt accession no.

Q14145

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... KEAP1(9817)

Specificity

Anti-Keap1 recognizes human Keap1.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product maybe stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
089K4806

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M T M van Jaarsveld et al.
Oncogene, 32(36), 4284-4293 (2012-10-10)
Epithelial ovarian cancer is the most lethal gynecological malignancy in the Western world. A major impediment for the successful treatment is the development of drug resistance. The molecular processes that contribute to resistance have been extensively studied; however, there is

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