K2876
Keratanase from Pseudomonas sp.
lyophilized powder
Synonym(s):
Endo-β-galactosidase
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
Recommended Products
form
lyophilized powder
packaging
vial of >10 units
storage temp.
−20°C
Unit Definition
One unit will liberate 1.0 μmole of reducing sugar (measured as galactose) from keratan sulfate per hr at pH 7.4 at 37°C.
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Kimberly M Anderson et al.
The Journal of biological chemistry, 280(9), 7720-7728 (2004-12-25)
We have isolated an endo-beta-galactosidase designated E-ABase from Clostridium perfringens ATCC 10543 capable of liberating both the A trisaccharide (A-Tri; GalNAcalpha1-->3(Fucalpha1-->2)Gal) and B trisaccharide (B-Tri; Galalpha1-->3(Fucalpha1-->2)Gal) from glycoconjugates containing blood group A and B glycotopes, respectively. We have subsequently cloned
Lu Deng et al.
Acta crystallographica. Section D, Biological crystallography, 60(Pt 3), 537-538 (2004-03-03)
The unique clostridial endo-beta-galactosidase (Endo-beta-Gal(GnGa)) capable of releasing the disaccharide GlcNAc alpha 1,4Gal from O-glycans expressed in the gastric gland mucous cell-type mucin has been crystallized. The crystal belongs to space group P6(3), with unit-cell parameters a = 160.4, c
Niclas G Karlsson et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 824(1-2), 139-147 (2005-08-02)
Capillary liquid chromatography-mass spectrometry using graphitised carbon stationary phase and ion trap mass spectrometry was shown to be a powerful technique for analysing glycosaminoglycans digested with endoglycosidases. Commonly found disaccharides from heparin/heparan sulphate digests at sub nanomole levels were found
Three-dimensional structure of GlcNAcalpha1-4Gal releasing endo-beta-galactosidase from Clostridium perfringens.
Wolfram Tempel et al.
Proteins, 59(1), 141-144 (2005-02-03)
William E B Johnson et al.
Arthritis and rheumatism, 46(10), 2658-2664 (2002-10-18)
To assess the effects of human intervertebral disc aggrecan on nerve growth and guidance, using in vitro techniques. Aggrecan extracted from human lumbar intervertebral discs was incorporated into tissue culture substrata for the culture of the human neuronal cell line
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