form
lyophilized powder
packaging
vial of >10 units
storage temp.
−20°C
Other Notes
One unit will liberate 1.0 μmole of reducing sugar (measured as galactose) from keratan sulfate per hr at pH 7.4 at 37°C.
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Yusuke Miki et al.
Xenotransplantation, 11(5), 444-451 (2004-08-12)
The presence of Galalpha1-3Galbeta1-4GlcNAc (alphaGal) in pigs is a formidable barrier for pig-to-primate xenotransplantation. We have reported that administration of recombinant endo-beta-galactosidase C (EndoGalC) removes alphaGal on porcine erythrocytes and kidneys. The present study examined the effects of EndoGalC gene
William E B Johnson et al.
Arthritis and rheumatism, 46(10), 2658-2664 (2002-10-18)
To assess the effects of human intervertebral disc aggrecan on nerve growth and guidance, using in vitro techniques. Aggrecan extracted from human lumbar intervertebral discs was incorporated into tissue culture substrata for the culture of the human neuronal cell line
Alternative strategy for overcoming ABO incompatibility.
Takaaki Kobayashi et al.
Transplantation, 83(9), 1284-1286 (2007-05-15)
Takeomi Murata et al.
European journal of biochemistry, 270(18), 3709-3719 (2003-09-03)
Novel chromogenic substrates for endo-beta-galactosidase were designed on the basis of the structural features of keratan sulfate. Galbeta1-4GlcNAcbeta1-3Galbeta1-4GlcNAcbeta-pNP (2), which consists of two repeating units of N-acetyllactosamine, was synthesized enzymatically by consecutive additions of GlcNAc and Gal residues to p-nitrophenyl
M N Fukuda
Current protocols in molecular biology, Chapter 17, Unit17-Unit17 (2008-02-12)
This overview covers the endo-beta-galactosidases; enzyme is capable of hydrolyzing a wide range of glycoconjugates. Endo-beta-galactosidases from numerous sources are discussed in terms of their substrate specificities and substrates, as well as their practical research applications.
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