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K0640

Sigma-Aldrich

Anti-Potassium Channel Kv11.1 (HERG) Extracellular−FITC antibody produced in rabbit

affinity isolated antibody, lyophilized powder

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Synonym(s):
Anti-KCNH2, Anti-Voltage gated K+ channel subfamily H member 2, Anti-ether-a-go-go-related channel 1
MDL number:
MFCD02097212
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

human

technique(s)

flow cytometry: 2-10 μg/mL using 1x106 human live cells
immunocytochemistry: 1:25 using intact live HEK-KV11.1 transfected cells

UniProt accession no.

Q12809

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... KCNH2(3757)

Related Categories

General description

Anti-Potassium Channel KV11.1 (HERG)- Extracellular-FITC (Voltage gated K+ channel subfamily H member 2, KCNH2, ether-a-go-go-related channel 1) is produced in rabbit using as immunogen the peptide AFLLKETEEGPPATEC corresponding to residues 430-445 of human KV11.1 (HERG). Anti-KV11.1 (HERG) antibody is directed against an extracellular epitope located between the S1 and S2 domains. The antibody is affinity purified on immobilized antigen and labeled with fluorescein isothiocyanate (FITC).
The gene Kv11.1, also referred to as KCNH2 (potassium voltage-gated channel subfamily H member 2), is mapped to human chromosome 7. The encoded voltage-activated potassium channel is a member of the ether-a-go-go (EAG) family.

Immunogen

peptide AFLLKETEEGPPATEC corresponding to residues 430-445 of human KV11.1 (HERG).

Application

Flow cytometry: a recommended working concentration of 2-10 μg per 1x106cells was determined using intact live human cells.

Biochem/physiol Actions

The gene Kv11.1, also referred to as HERG (human ether-a-go-go related gene), encodes the α-subunit of the rapid component of the delayed rectifier potassium channel. The third repolarization of the action potential of human cardiomyocytes is carried out by this protein. Mutations in this gene have been associated with type II hereditary long QT syndrome (LQT2). This disease is characterized by prolongation of the QT interval, abnormal T wave, torsade de pointes, syncope and sudden death caused by cardiac arrest.

Physical form

Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide.

Reconstitution

Reconstitute the lyophilized vial with 50 μL or 200 μL deionized water, depending on package size. Further dilutions should be made using a carrier protein such as BSA (1-3%).

Other Notes

Lyophilized powder can be stored intact at room temperature for several weeks. For extended storage, it should be stored at -20 °C or below. The reconstituted solution can be stored at 2-8 °C for up to 2 weeks. For longer storage, freeze in working aliquots. Avoid repeated freezing and thawing. Storage in “frost-free” freezers is not recommended. Centrifuge before use. Working dilution samples should be discarded if not used within 12 hours. The antibody is stable for at least 12 months when stored appropriately.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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  5. What is the concentration of Product K0640, Anti-Potassium Channel Kv11.1 (HERG) Extracellular - FITC antibody produced in rabbit after reconstitution with 50 ul of water?

    Product K0640, Anti-Potassium Channel Kv11.1 (HERG) Extracellular - FITC antibody produced in rabbit will have a concentration of 1 mg/ml when reconstituted with 50 ul of water.

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Yu-Wen Cheng et al.
International journal of molecular sciences, 24(16) (2023-08-26)
KCNH2 loss-of-function mutations cause long QT syndrome type 2 (LQT2), an inherited cardiac disorder associated with life-threatening ventricular arrhythmia. Through whole-exome sequencing, we discovered a novel AGCGACAC deletion (S981fs) in the hERG gene of an LQT2 patient. Using a heterologous
Aigul Moldobaeva et al.
Angiogenesis, 20(1), 73-84 (2016-10-28)
Although bronchial angiogenesis has been well documented in allergic asthma, lymphangiogenesis has not been widely studied. Therefore, we evaluated changes in lung lymphatics in a rat model of allergen-induced asthma using house dust mite (Der p 1; 100 μg/challenge). Additionally, properties
[Progress in research on defective protein trafficking and functional restoration in HERG-associated long QT syndrome].
Fang P and Lian J
Zhonghua yi xue yi chuan xue za zhi (Chinese Journal of Medical Genetics), 33, 101-104 (2016)
Xianbin Zhang et al.
Frontiers in oncology, 8, 590-590 (2018-12-21)
In this study we evaluated the interaction of pancreatic cancer cells, cancer-associated fibroblasts, and distinct drugs such as α-cyano-4-hydroxycinnamate, metformin, and gemcitabine. We observed that α-cyano-4-hydroxycinnamate as monotherapy or in combination with metformin could significantly induce collagen I deposition within

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