ISO2
Mouse Monoclonal Antibody Isotyping Reagents
sufficient for 1000 tests (clones) (by ELISA), sufficient for 40 tests (clones) (by immunodiffusion, ODD)
Synonym(s):
Mouse Antibody Isotyping Kit
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About This Item
usage
sufficient for 1000 tests (clones) (by ELISA)
sufficient for 40 tests (clones) (by immunodiffusion, ODD)
Quality Level
shipped in
wet ice
storage temp.
2-8°C
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General description
The Mouse Monoclonal Antibody Isotyping Reagents enable qualitative isotype determination of mouse monoclonal antibosies derived from hybridoma supernatant, ascites fluid or purified forms.
Specificity
ISO-2 contains 0.2 mL vials of subclass specific antibodies to all four mouse IgG subclasses, IgA, and IgM. Directions are included for using these reagents in ELISA and Ouchterlony assays to determine the subclass of monoclonal antibodies in ascites, culture supernatants, or purified preparations. Additional reagents may be required, such as conjugates for ELISA assays or agarose and buffer components for Ouchterlony assays.
Application
Mouse Monoclonal Antibody Isotyping Reagents has been used in monoclonal antibody isotype determination using enzyme-linked immunosorbent assay (ELISA). It may be used in Ouchterlony immunodiffusion assays.
Determination of the subclass of a monoclonal antibody is helpful for characterization of the antibody, for choosing detection reagents, and for deciding on a purification scheme.
Features and Benefits
- May be used in a variety of assay formats
- Suitable for all antibody forms
- Determines all mouse IgG subclasses, IgA, and IgM
Kit Components Only
Product No.
Description
- goat antisera to mouse IgA .2 mL
- goat antisera to mouse IgG3 .2 mL
- goat antisera to mouse IgG1 .2 mL
- goat antisera to mouse IgG2a .2 mL
- goat antisera to mouse IgG2b .2 mL
- goat antisera to mouse IgM .2 mL
related product
Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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Preparation and Characterization of a Monoclonal Antibody with High Affinity for Soluble A β Oligomers
Ying,Z et al.
Hybridoma, 28(5) (2009)
Zhang Ying et al.
Hybridoma (2005), 28(5), 349-354 (2009-10-28)
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Journal of hypertension, 26(12), 2414-2425 (2008-11-15)
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Masanori Onda et al.
Journal of immunology (Baltimore, Md. : 1950), 177(12), 8822-8834 (2006-12-05)
Recombinant immunotoxins composed of an Ab Fv fragment joined to a truncated portion of Pseudomonas exotoxin A (termed PE38) have been evaluated in clinical trials for the treatment of various human cancers. Immunotoxin therapy is very effective in hairy cell
Protocols
Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.
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