Skip to Content
Merck
CN
All Photos(3)

Key Documents

View All Documentation

Safety Information

I9392

Sigma-Aldrich

3-Methylbutanol

BioReagent, ≥98.5%, Molecular Biology

Synonym(s):

3-Methyl-1-butanol, Isoamyl alcohol, Isopentyl alcohol

Sign Into View Organizational & Contract Pricing

Select a Size

All Photos(3)

Select a Size

Change View

About This Item

Linear Formula:
(CH3)2CHCH2CH2OH
CAS Number:
123-51-3
Molecular Weight:
88.15
Beilstein:
1718835
EC Number:
204-633-5
MDL number:
MFCD00002934
UNSPSC Code:
12352200
PubChem Substance ID:
24896157
NACRES:
NA.52
grade:
Molecular Biology
technique(s):
RNA extraction: suitable

grade

Molecular Biology

Quality Level

200

product line

BioReagent

Assay

≥98.5%

form

liquid

technique(s)

RNA extraction: suitable

pH

5.6 (20 °C, 25 g/L)

density

0.809 g/mL at 20 °C (lit.)

suitability

suitable for nucleic acid purification

storage temp.

room temp

Looking for similar products? Visit Product Comparison Guide

General description

3-methylbutanol (isoamyl alcohol) is a clear, colorless alcohol commonly used as an organic solvent . It can also be used in phenol-chloroform nucleic acid extraction and purification protocols, where it inhibits RNase activity and reduces foaming. Isoamyl alcohol can also be used for the removal of ethidium bromide from DNA purified by CsCl gradient ultracentrifugation.

Application

3-Methylbutanol (isoamyl alcohol) in combination with phenol and chloroform is used in extraction of RNA. It was used in the synthesis of oligo-RNAs using photocaged adenosine molecules.[1]
3-Methylbutanol has been used as a component of phenol/chloroform/ isoamyl alcohol solution for DNA extraction from blood and from absorbing substrata based forensic samples[2] and biofilms.[3]

related product

Product No.
Description
Pricing

Signal Word

Danger

Hazard Statements

H226,H315,H318,H332,H335

Hazard Classifications

Acute Tox. 4 Inhalation - Eye Dam. 1 - Flam. Liq. 3 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

110.3 °F - closed cup

Flash Point(C)

43.5 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

危险化学品
  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    SHBS6029
    SHBP7087
    SHBL4025
    SHBK7144
    SHBK6074

    Don't see the Right Version?

    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

    Search for a COA

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    DNA extraction from blood and forensic samples
    Guidelines for Molecular Analysis in Archive Tissues, 45-54 (2011)
    Section 1 Isolation of nucleic acids
    Biofouling Methods, 88-88 (2014)
    Steven G Chaulk et al.
    Nature protocols, 2(5), 1052-1058 (2007-06-05)
    This protocol describes a general method for the preparation of RNAs in which the reactivity or hydrogen-bonding properties of the molecule are modified in a photoreversible fashion by use of a caging strategy. A single caged adenosine, modified at the
    Mary J Dunlop et al.
    Molecular systems biology, 7, 487-487 (2011-05-11)
    Many compounds being considered as candidates for advanced biofuels are toxic to microorganisms. This introduces an undesirable trade-off when engineering metabolic pathways for biofuel production because the engineered microbes must balance production against survival. Cellular export systems, such as efflux
    Jane Melville et al.
    PloS one, 9(7), e101847-e101847 (2014-07-31)
    Molecular phylogenetics is increasingly highlighting the prevalence of cryptic species, where morphologically similar organisms have long independent evolutionary histories. When such cryptic species are known to be declining in numbers and are at risk of extinction due to a range
    View All Related Papers

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service