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I7403

Sigma-Aldrich

L-Isoleucine

98.5-101.0%, suitable for cell culture, BioXtra, non-animal source

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Synonym(s):
(2S,3S)-2-Amino-3-methylpentanoic acid
Linear Formula:
C2H5CH(CH3)CH(NH2)CO2H
CAS Number:
73-32-5
Molecular Weight:
131.17
Beilstein:
1721792
EC Number:
200-798-2
MDL number:
MFCD00064222
UNSPSC Code:
12352209
eCl@ss:
32160406
PubChem Substance ID:
24896115
NACRES:
NA.26

biological source

non-animal source

Quality Level

300

Agency

USP/NF
meets EP testing specifications
meets JP testing specifications
meets USP testing specifications

product line

BioXtra

Assay

98.5-101.0%

form

powder

quality

meets EP, JP, USP testing specifications

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

white

mp

288 °C (dec.) (lit.)

solubility

1 M HCl: 50 mg/mL

application(s)

peptide synthesis
pharmaceutical (small molecule)

SMILES string

CC[C@H](C)[C@H](N)C(O)=O

InChI

1S/C6H13NO2/c1-3-4(2)5(7)6(8)9/h4-5H,3,7H2,1-2H3,(H,8,9)/t4-,5-/m0/s1

InChI key

AGPKZVBTJJNPAG-WHFBIAKZSA-N

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Application

L-Isoleucine has been used as a supplement of maintenance media consisting of DMEM/F-12 for the culture of primary hepatocytes. It has also been used as a supplement for serial culture of adult murine keratinocytes.

Biochem/physiol Actions

L-Isoleucine is an essential amino acid that is synthesized from threonine. It is a branched-chain hydrophobic amino acid. L-isoleucine is an isomer of L-leucine.

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Inhibitory effect of aroma on the bitterness of branched-chain amino acid solutions.
Mukai J
Chemical & Pharmaceutical Bulletin, 55(11), 1581-1584 (2007)
Biochemistry (5th Edition) null
Conversion of ammonia or urea into essential amino acids,L-leucine,L-valine, andL-isoleucine using artificial cells containing an immobilized multienzyme system and dextran-NAD+
Kang Fu Gu
Applied Biochemistry and Biotechnology, 26 (1990)
Recapitulation of metabolic defects in a model of propionic acidemia using patient-derived primary hepatocytes.
Chapman KA
Molecular Genetics and Metabolism, 117(3), 355-362 (2016)
Serial Cultivation of Primary Adult Murine Keratinocytes
Richard P. Redvers and Pritinder Kaur
Methods in Molecular Biology (2005)
View All Related Papers

Chromatograms

application for HPLC

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