I7153
Anti-Insulin Receptor Substrate 1 (IRS-1) antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
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biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 165 kDa
species reactivity
human, mouse, rat
technique(s)
immunoprecipitation (IP): 4 μg using 0.5 mg of a 3T3/A31 RIPA lysate
western blot: 0.5-2 μg/mL using RIPA lysates of 3T3/A31 mouse fibroblasts
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... IRS1(3667)
mouse ... Irs1(16367)
rat ... Irs1(25467)
General description
Insulin receptor substrate 1 (IRS1) gene is mapped to human chromosome 2q36.3. The gene encodes a 185 kDa protein and is ubiquitously expressed. Irs1 belongs to the group of cytoplasmic adaptor proteins.
Immunogen
C-terminal 14 amino acid residues of rat liver insulin receptor substrate 1.
Application
Anti-Insulin Receptor Substrate 1 (IRS-1) antibody can be used in immunoprecipitation using 0.5 mg of a 3T3/A31 RIPA lysate.
Biochem/physiol Actions
Insulin receptor substrate 1 (IRS-1) is phosphorylated on multiple tyrosine residues. It plays a crucial role in transmitting signals from the insulin and insulin-like growth factor-1 (IGF-1) receptors to intracellular pathways. Over expression of IRS-1can leads to mammary tumorigenesis and metastasis. Anti-Insulin Receptor Substrate 1 (IRS-1) antibody can be used in western blotting using RIPA lysates of 3T3/A31 mouse fibroblasts. Rabbit anti-Insulin Receptor Substrate 1 (IRS-1) antibody reacts specifically with insulin receptor substrate 1 (165 kD). The product has shown species cross reactivity with rat, mouse and human IRS-1.
Physical form
Solution in 0.1 M Tris-glycine, pH 7.4, containing 0.15 M NaCl, and 0.05% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
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S R Keller et al.
Molecular reproduction and development, 35(4), 346-351 (1993-08-01)
The insulin and insulin-like growth factor-I (IGF-I) receptors are tyrosine kinases. Consequently, an approach to investigating signaling pathways from these receptors is to characterize proteins rapidly phosphorylated on tyrosine in response to insulin and IGF-I. In many cell types the
The insulin receptor substrate (IRS) proteins: at the intersection of metabolism and cancer
Shaw LM
Cell Cycle, 10(11), 1750-1756 (2011)
Common variants in and near IRS1 and subclinical cardiovascular disease in the Framingham Heart Study
Lim S, et al.
Atherosclerosis, 229(1), 149-154 (2013)
Robert K Dearth et al.
Molecular and cellular biology, 26(24), 9302-9314 (2006-10-13)
Insulin receptor substrates (IRSs) are signaling adaptors that play a major role in the metabolic and mitogenic actions of insulin and insulin-like growth factors. Reports have recently noted increased levels, or activity, of IRSs in many human cancers, and some
Judit Mohás-Cseh et al.
Biomedicines, 10(5) (2022-05-29)
A link between oxidative stress and insulin resistance has been suggested. Hydroxyl free radicals are known to be able to convert phenylalanine (Phe) into the non-physiological tyrosine isoforms ortho- and meta-tyrosine (o-Tyr, m-Tyr). The aim of our study was to
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