I4753
Invertase from Candida utilis
Grade X, ≥300 units/mg solid
Synonym(s):
β-D-Fructofuranoside fructohydrolase, β-Fructofuranosidase, Saccharase
type
Grade X
Quality Level
form
solid
specific activity
≥300 units/mg solid
mol wt
~260 kDa
foreign activity
α-galactosidase (melibiase), essentially free
storage temp.
−20°C
Related Categories
General description
Invertase from Candida utilis is secreted in the periplasm. The molecular weight of invertase from C. utilis is 300 kDa. It is a dimer of identical subunits. It is a N-glycosylated protein, rich in mannose oligosaccharide in its structure. The invertase encoding gene of C. utilis shares high homology with SUC2 gene of Saccharomyces cerevisiae.
Michaelis constant : 1.5x10 -2M (Saccharose)
Structure : Glycoprotein containing ca. 50% of carbohydrates
Optimum pH : 3.5 - 4.0 Optimum temperature : 60 - 70oC
pH Stability : pH 4.0 - 6.0 (50oC, 10min)
Thermal stability : below 60oC (pH 4.5, 10min)
Substrate specificity : The enzyme hydrolyzes saccharose and raffinose, but does not
hydrolyze inulin and melezitose.6)
Structure : Glycoprotein containing ca. 50% of carbohydrates
Optimum pH : 3.5 - 4.0 Optimum temperature : 60 - 70oC
pH Stability : pH 4.0 - 6.0 (50oC, 10min)
Thermal stability : below 60oC (pH 4.5, 10min)
Substrate specificity : The enzyme hydrolyzes saccharose and raffinose, but does not
hydrolyze inulin and melezitose.6)
Application
Invertase from Candida utilis has been used:
- as a supplement in YE liquid medium to observe for morphological changes in Streptomyces sp.
- to determine the sucrose content in ethanolic extract of leaf tissues
- to determine the total non-structural carbohydrates (TNC) in root tissues
This enzyme is useful for enzymatic determination of saccharose and for the structure investigation of carbohydrates containing β-D-fructofuranoside residue.
Biochem/physiol Actions
Invertase acts on sucrose and cleaves it into glucose and fructose. It is used in the preparation of invert syrup with equimolar concentration of fructose and sucrose. It finds its applications in confectionaries and in the production of alcoholic beverages, glycerol and lactic acid. Invertase is also used in the preparation of artificial honey, plasticizers for cosmetic industries.
Preparation Note
Chromatographically purified
Other Notes
One unit will hydrolyze 1.0 μmole of sucrose to invert sugar per min at pH 4.5 at 55°C.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Invertase and its applications-a brief review
Kulshrestha S, et al.
Journal of Pharmacy Research, 7(9), 792-797 (2013)
Purification and characterization of an invertase from Candida utilis: comparison with natural and recombinant yeast invertases
Chavez FP, et al.
Journal of Biotechnology, 53(1), 67-74 (1997)
Interactive effects of elevated carbon dioxide and growth temperature on photosynthesis in cotton leaves
Reddy AR, et al.
Plant Growth Regulation, 26(1), 33-40 (1998)
Morphological and physiological changes in Streptomyces lividans induced by different yeasts
Santamaria R, et al.
Archives of Microbiology, 177(3), 259-266 (2002)
Winter foot and equipment traffic impacts on a `L93?creeping bentgrass putting green
Baldwin CM, et al.
Hortscience: a Publication of the American Society For Horticultural Science Hortscience, 43(3), 922-926 (2008)
Protocols
The appearance of reducing sugars is measured by the modified Fehling-Lehmann-Schoorl method.
Enzymatic Assay of Invertase
在测定转化酶活性时，使用了一种在410nm处的分光光度终点反应法进行测定。在55 ºC、pH 4.5条件下，一单位的转化酶每分钟可将1.0 μmole的蔗糖水解为转化糖。
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