I3642
Monoclonal Anti-Interleukin-1β antibody produced in mouse
clone 8516.311, purified immunoglobulin, lyophilized powder
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Synonym(s):
Anti-IL-1β
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
8516.311, monoclonal
form
lyophilized powder
species reactivity
human
technique(s)
flow cytometry: suitable
immunohistochemistry: 8-25 μg/mL using human PBMC′s
neutralization: suitable
western blot: 1 μg/mL
isotype
IgG1
UniProt accession no.
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... IL1B(3553)
Related Categories
Specificity
The antibody will neutralize the biological activity of recombinant human IL-1β. It will not neutralize the activity of recombinant human IL-α, recombinant mouse IL-1α, or recombinant mouse IL-1β. When immobilized on a microplate, this antibody will capture both recombinant as well as natural human IL-1β. In immunoblotting, the antibody shows ~25% cross-reactivity with recombinant rat IL-1β and <5% cross-reactivity with recombinant mouse IL-1β and recombinant porcine IL-1β. Additionally, in immunoblotting, the antibody exhibits no cross-reactivity with recombinant mouse IL-α, recombinant human IL-1ra, recombinant mouse IL-1ra, or recombinant rat IL-1α.
Immunogen
purifed, E. coli-derived, recombinant human IL-1β.
Application
May used in immunoblotting, immunohistochemistry, ELISA, and neutralization assays
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.
Preparation Note
Monoclonal Anti-Interleukin-1(IL-1) (mouse IgG1 isotype) is purified from a mouse hybridoma utilizing Protein A affinity chromatography.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Certificates of Analysis (COA)
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The impairment of the cystic fibrosis transmembrane conductance regulator (CFTR) activity induces intracellular chloride (Cl- ) accumulation. The anion Cl- , acting as a second messenger, stimulates the secretion of interleukin-1β (IL-1β), which starts an autocrine positive feedback loop. Here
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