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Safety Information

I3146

Sigma-Aldrich

ITS Liquid Media Supplement (100×)

liquid, sterile-filtered, BioReagent, suitable for cell culture

Synonym(s):

Human ITS Liquid Media, Insulin-transferrin-sodium selenite

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100 G
CN¥758.56
250 G
CN¥1,541.14

CN¥758.56

Estimated to ship onMay 06, 2025Details

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100 G
CN¥758.56
250 G
CN¥1,541.14

About This Item

UNSPSC Code:
12352205
NACRES:
NA.75

CN¥758.56

Estimated to ship onMay 06, 2025Details

Request a Bulk Order

sterility

sterile-filtered

Quality Level

500

product line

BioReagent

form

liquid

technique(s)

cell culture | mammalian: suitable
single cell analysis: suitable

impurities

endotoxin, tested

shipped in

ambient

storage temp.

2-8°C

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This Item
285740100684PHR1419
Sulfanilic acid GR for analysis ACS,Reag. Ph Eur

100686

Sulfanilic acid

磺胺酸 JIS special grade, 99.0-100.5%

285740

磺胺酸

Sulfanilic acid for elemental analysis

100684

Sulfanilic acid

对氨基苯磺酸 Pharmaceutical Secondary Standard; Certified Reference Material

PHR1419

对氨基苯磺酸

grade

ACS reagent

grade

JIS special grade

grade

-

grade

certified reference material, pharmaceutical secondary standard

assay

≥99.0-102.0% (acidimetric)

assay

99.0-100.5%

assay

-

assay

-

Quality Level

100

Quality Level

-

Quality Level

100

Quality Level

300

agency

reag. Ph. Eur.

agency

-

agency

-

agency

traceable to USP 1633506

form

solid

form

powder

form

solid

form

-

storage temp.

15-25°C

storage temp.

-

storage temp.

15-25°C

storage temp.

2-30°C

Application

ITS Liquid Media Supplement (100×) has been used as a media supplement to culture:
  • α mouse liver 12 (AML-12) cells[1]
  • rat fetal testis explants[2]
  • human dental pulp stem cells (DPSC)[3]

Biochem/physiol Actions

  • Insulin: a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe an observed mitogenic effect to this property.[4]
  • Transferrin: an iron-transport protein.
  • Selenium: an essential trace element normally provided by serum.[5]

Components

Contains 1.0 mg/ml recombinant human insulin, 0.55 mg/ml human transferrin (substantially iron-free), and 0.5 μg/ml sodium selenite at the 100x concentration.

Other Notes

ITS is a mixture of recombinant human insulin, human transferrin, and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g. MEM, RPMI-1640) and complex media (e.g. Ham?s F-12, DME/F-12, MEM) with sodium pyruvate.

Preparation Note

Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品
  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000366647
    0000366647
    0000359154
    0000346703
    0000269673

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    Find documentation for the products that you have recently purchased in the Document Library.

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    Leonardo Ricotti et al.
    Biomedical materials (Bristol, England), 6(3), 031001-031001 (2011-05-14)
    Polyelectrolyte layer-by-layer (LbL) nanofilms are interesting polymeric structures, built by alternating adsorption of positively and negatively charged polyelectrolytes. They consist of multilayer sheets with nanometric overall thickness, and they can be used as supports and surface coatings for in vitro
    Christine M Reich et al.
    Veterinary research communications, 36(2), 139-148 (2012-03-07)
    In the dog, mesenchymal stem cells (MSCs) have been shown to reside in the bone marrow (bone marrow-derived mesenchymal stem cells: BM-MSCs) as well as in the adipose tissue (adipose tissue-derived stem cells: ADSCs). Potential application fields for these multipotent
    Zhibin Lin et al.
    Cell death & disease, 11(1), 58-58 (2020-01-25)
    Translationally controlled tumor protein (TCTP), which is a protein characterized by its potent proliferation promoting activity, has been well studied in the area of growth and tumorigenesis. However, the specific role of TCTP in liver regeneration (LR) and its underlying
    Yi Yu et al.
    Scientific reports, 7(1), 1729-1729 (2017-05-13)
    Hepatitis B virus (HBV) infection causes acute hepatitis B (AHB), chronic hepatitis B (CHB), liver cirrhosis (LC), and eventually hepatocellular carcinoma (HCC). The presence of hepatitis B e antigen (HBeAg) in the serum generally indicates ongoing viral replication and disease
    Kelvin S C Cheung et al.
    PloS one, 9(6), e98063-e98063 (2014-06-04)
    MicroRNAs (miRs) play a pivotal role in a variety of biological processes including stem cell differentiation and function. Human foetal femur derived skeletal stem cells (SSCs) display enhanced proliferation and multipotential capacity indicating excellent potential as candidates for tissue engineering
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    Articles

    Selenium in Cell Culture

    Understand the role of selenium in cell culture media formulations. The Media Expert library explains how culture media ingredients support cell growth.

    Transferrin in Cell Culture

    Understand how transferrin affects serum-free cell culture systems, crucial for biomanufacturing heterologous proteins like monoclonal antibodies.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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