I2002
Isocitric Dehydrogenase (NADP) from porcine heart
Type IV, buffered aqueous glycerol solution, 3-20 units/mg protein
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type
Type IV
Quality Level
form
buffered aqueous glycerol solution
quality
≤ 0.2% of main activity is obtained with NAD as cofactor.
specific activity
3-20 units/mg protein
composition
Protein biuret
shipped in
wet ice
storage temp.
2-8°C
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Application
Isocitric dehydrogenase (IDH) has been used:
- for in vitro activity assay to study the effects of thorium on citric acid cycle enzymes in the mitochondrial respiration pathway
- as a standard to measure the impact of temperature on nicotinamide diphosphate (NADP)-IDH activity
- in isocitrate assay
Biochem/physiol Actions
Isocitrate dehydrogenase (IDH) is a homodimeric enzyme and participates in several processes such as adapting to hypoxia, histone demethylation, and DNA modification. This enzyme has three isoforms like IDH1, IDH2, and IDH3.
Unit Definition
One unit will convert 1.0 μmole of isocitrate to α-ketoglutarate per min at pH 7.4 at 37 °C.
Physical form
Solution in 50% glycerol in EDTA buffer salts, pH 6.0
inhibitor
Product No.
Description
Pricing
WGK
WGK 2
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
动植物源性产品
Certificates of Analysis (COA)
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Intracellular iron levels are strictly regulated to support homeostasis and avoid iron-mediated ROS production. Loss of iron-sulfur cluster (ISC) synthesis can increase iron loading and promote cell death by ferroptosis. Iron-responsive element-binding proteins IRP1 and IRP2 posttranscriptionally regulate iron homeostasis.
Icksoo Lee et al.
Biochimica et biophysica acta, 1802(2), 275-283 (2009-10-20)
Noonan syndrome (NS) is an autosomal dominant disorder, and a main feature is congenital heart malformation. About 50% of cases are caused by gain-of-function mutations in the tyrosine phosphatase SHP2/PTPN11, a downstream regulator of ERK/MAPK. Recently it was reported that
Avtar Singh et al.
The Journal of biological chemistry, 278(35), 33208-33216 (2003-06-07)
Transhydrogenase couples the reduction of NADP+ by NADH to inward proton translocation across mitochondrial and bacterial membranes. The coupling reactions occur within the protein by long distance conformational changes. In intact transhydrogenase and in complexes formed from the isolated, nucleotide-binding
M Ryan Smith et al.
Redox biology, 8, 136-148 (2016-01-18)
Many cancer cells follow an aberrant metabolic program to maintain energy for rapid cell proliferation. Metabolic reprogramming often involves the upregulation of glutaminolysis to generate reducing equivalents for the electron transport chain and amino acids for protein synthesis. Critical enzymes
NADP +-dependent isocitrate dehydrogenase activity in marine plankton
Tames Espinosa M, et al.
Marine Chemistry, 204, 86-94 (2018)
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