I0516
Insulin solution from bovine pancreas
10 mg/mL insulin in 25 mM HEPES, pH 8.2, BioReagent, sterile-filtered, suitable for cell culture
Synonym(s):
Bovine insulin, Cell culture grade insulin
About This Item
Recommended Products
sterility
sterile-filtered
Quality Level
product line
BioReagent
form
solution
concentration
10 mg/mL insulin in 25 mM HEPES, pH 8.2
technique(s)
cell culture | mammalian: suitable
single cell analysis: suitable
impurities
endotoxin, tested
pH
7.9-8.5
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
SMILES string
SC[C@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CN)[C@H](CC)C)C(C)C)CCC(=O)O)CCC(=O)N)CS)C(=O)N[C@@H](C)C(=O)N[C@@H](CO)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CS)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc4ccc(cc4)O)C(=O)N[
InChI
1S/2C97H151N25O34S4/c2*1-12-46(10)77(120-72(131)35-98)96(154)122-76(45(8)9)94(152)107-55(24-28-74(134)135)79(137)104-53(22-26-69(100)128)82(140)116-66(40-159)93(151)118-64(38-157)90(148)103-47(11)78(136)114-63(37-124)89(147)121-75(44(6)7)95(153)119-67(41-160)92(150)115-62(36-123)88(146)109-57(30-43(4)5)84(142)110-58(31-48-13-17-50(125)18-14-48)85(143)105-52(21-25-68(99)127)80(138)108-56(29-42(2)3)83(141)106-54(23-27-73(132)133)81(139)112-60(33-70(101)129)87(145)111-59(32-49-15-19-51(126)20-16-49)86(144)117-65(39-158)91(149)113-61(97(155)156)34-71(102)130/h2*13-20,42-47,52-67,75-77,123-126,157-160H,12,21-41,98H2,1-11H3,(H2,99,127)(H2,100,128)(H2,101,129)(H2,102,130)(H,103,148)(H,104,137)(H,105,143)(H,106,141)(H,107,152)(H,108,138)(H,109,146)(H,110,142)(H,111,145)(H,112,139)(H,113,149)(H,114,136)(H,115,150)(H,116,140)(H,117,144)(H,118,151)(H,119,153)(H,120,131)(H,121,147)(H,122,154)(H,132,133)(H,134,135)(H,155,156)/t2*46-,47-,52-,53-,54-,55-,56-,57-,58-,59-,60-,61-,62-,63-,64-,65-,66-,67-,75-,76-,77-/m00/s1
InChI key
XYPANFGXFLOTDK-CJLNJPJHSA-N
Gene Information
bovine ... INS(280829)
cow ... INS(280829)
Application
- to study the effect of exogenous insulin on plasma concentrations of glucose, insulin, insulin-like growth factor I (IGF-I), and progesterone (P4) in pubertal beef heifers
- to study the insulin responsiveness of the placenta during pregnancy complications using primary human trophoblast cells and placental villous explants
- to study the effect of insulin receptor signaling dendritic spine density in neonatal mouse hippocampal neurons in vitro
Biochem/physiol Actions
Physical form
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Regulatory Information
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Certificates of Analysis (COA)
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
What is the comparison between International Units (IU) and USP units for insulin?
The international unit (IU) of insulin is based on the International Standard Preparation of Bioassay (1958). The standard preparation is a mixture of 52% bovine and 48% porcine insulin, containing 24 units/mg. Reference: Martindale: The Extra Pharmacopoeia, 29th ed., p. 392. High purity bovine pancreatic insulin typically assays at approximately 27 USP units per mg. So the IU and USP unit are not identical, but they are approximately 1:1.
What is the amino acid sequence for insulin?
The sequence and structure of insulin can be found on the product information sheet (under Documents, above).
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How many units/mg am I getting in Product I0516, Insulin solution?
This solution is made using insulin Product No. I5500. The potency specification for the I5500 is not less than 27 USP units/mg. However, we do not test for potency of the final product of this solution. It is possible some activity is lost during the manufacturing process.
What is the molarity of Product I0516, Insulin solution?
Product No. I0516 is a 10 mg/mL solution of bovine insulin (MW 5733.5). The molarity is 1.7 μmol/mL or 1.7 mM.
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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