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Key Documents

Safety Information

HT20

Sigma-Aldrich

Iron Stain

Synonym(s):

Prussian Blue Test Kit

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About This Item

UNSPSC Code:
41116121
NACRES:
NA.47

form

solution

Quality Level

shelf life

Expiry date on the label.

IVD

for in vitro diagnostic use

application(s)

hematology
histology

storage temp.

room temp

Principle

Deposits of loosely bound iron in erythrocytes, bone marrow, or tissue stain an intense blue when treated with acid ferrocyanide according to the well-known Prussian blue reaction.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Carc. 1B - Flam. Liq. 2 - Met. Corr. 1 - STOT SE 1

Target Organs

Eyes,Central nervous system

Storage Class Code

3 - Flammable liquids

Flash Point(F)

51.8 °F

Flash Point(C)

11 °C

Regulatory Information

监管及禁止进口产品

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Certificates of Analysis (COA)

Lot/Batch Number

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Can I buy the reagents in Iron Stain Kit, Product HT20, separately?

    The reagents included in this kit are not available for separate purchase.

  4. Is EDTA an acceptable anticoagulant for use with the Iron Stain Kit, Product HT20?

    Blood and bone marrow samples should be drawn without the use of EDTA as an anticoagulant. The EDTA chelates enough Iron to interfere with adequate staining. We advise using heparinized blood or freshly drawn blood which has not been anticoagulated.

  5. Is it necessary to use the counterstain to view tissue Iron when using the Iron Stain Kit, Product HT20?

    Iron in tissue samples (used for research) can be visualized without the Pararosaniline counterstain, which is used to allow improved visualization of the tissue.

  6. Can the Iron Stain Kit, Product HT20, be used to stain cultured cells, rather than blood or bone marrow?

    This kit can be used to stain cultured mammalian cells, which should be treated as blood or bone marrow and follow instructions given for specimen collection in the Package Insert.  We advise using some sort of positive control.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Carl Maximilian Thielmann et al.
Scientific reports, 9(1), 11326-11326 (2019-08-07)
Iron-loaded tumor-associated macrophages (iTAMs) show a pro-inflammatory phenotype, hallmarked by anti-tumorigenic activity and an ability to attenuate tumor growth. Here we explored the relevance of these findings in lung cancer patients by investigating the impact of the iTAM content in
J Venugopal et al.
Scientific reports, 10(1), 16514-16514 (2020-10-07)
Proprotein convertase subtilisin/kexin type 9 (PCSK9) deficiency leads to lower cholesterol and is associated with reduced vascular complications in the general population. Cholesterol lowering may also have beneficial effects in sickle cell disease (SCD). The objective of this study was
Jessica Cooperrider et al.
Experimental neurology, 277, 27-34 (2015-12-25)
The olivo-cerebellar system has an essential role in the detection and adaptive correction of movement errors. While there is evidence of an error signal in the cerebellar cortex and inferior olivary nucleus, the deep cerebellar nuclei have been less thoroughly
James Bouley et al.
Journal of neurotrauma, 36(7), 1008-1017 (2018-07-13)
Cortical spreading depression (CSD) has been described after moderate-to-severe traumatic brain injury (TBI). It is uncertain, however, whether CSD occurs after mild, concussive TBI and whether it relates to brain pathology and functional outcome. Male C57BL6/J mice (n = 62) were subjected
Renu John et al.
Proceedings of the National Academy of Sciences of the United States of America, 107(18), 8085-8090 (2010-04-21)
Dynamic magnetomotion of magnetic nanoparticles (MNPs) detected with magnetomotive optical coherence tomography (MM-OCT) represents a new methodology for contrast enhancement and therapeutic interventions in molecular imaging. In this study, we demonstrate in vivo imaging of dynamic functionalized iron oxide MNPs

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