HT101128
Bouin′s solution
histological fixative
Synonym(s):
Bouin’s fluid
Product Name
Bouin′s solution, histological fixative
shelf life
Expiry date on the label
composition
acetic acid, 5%
formaldehyde, 9%
picric acid, 0.9%
color
yellow
application(s)
hematology
histology
storage temp.
room temp
Quality Level
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Application
Bouin′s solution has been used:
- in the staining of human liver biopsy tissue samples for two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) imaging
- in the fixation of rat ovaries for the observing follicle cells using microscopy
- in the fixation of gonads of crab for morphological and histological analysis
Prepared with saturated picric acid, formaldehyde and acetic acid. Excellent fixative for preserving soft and delicate structures. Used as a mordant in various trichrome procedures.
General description
Bouin′s solution is a noncoagulant yellow stain fixative. It imposes shrinkage on tissues when used as preservative due to its picric acid component. It is majorly used in ureteroscopic biopsies. It preserves morphological and nuclear information in tissues upon fixation.
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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Improving the interpretation of ureteroscopic biopsies: use of Bouin's fixative
Bultitude MF, et al.
BJU International, 108(9), 1373-1375 (2011)
Nonlinear optical microscopy: use of second harmonic generation and two-photon microscopy for automated quantitative liver fibrosis studies
Sun W, et al.
Journal of Biomedical Optics, 13(6), 064010-064010 (2008)
DNA and RNA obtained from Bouin?s fixed tissues
Bonin S, et al.
Journal of Clinical Pathology, 58(3), 313-316 (2005)
Testis Maturation Stages of Mud Crab (Scylla olivacea) Broodstock on Different Diets
Ikhwanuddin M, et al.
Sains Malaysiana, 47(3), 427-432 (2018)
Maternal undernutrition significantly impacts ovarian follicle number and increases ovarian oxidative stress in adult rat offspring
Bernal AB, et al.
Testing, 5(12), e15558-e15558 (2010)
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