Skip to Content
Merck
CN
All Photos(1)

Documents

HT101128

Sigma-Aldrich

Bouin′s solution

histological fixative

Sign Into View Organizational & Contract Pricing
All Photos(1)
Synonym(s):
Bouin’s fluid
MDL number:
MFCD00146169
UNSPSC Code:
12352200
NACRES:
NA.47

Quality Level

200

shelf life

Expiry date on the label

composition

acetic acid, 5%
formaldehyde, 9%
picric acid, 0.9%

color

yellow

application(s)

hematology
histology

storage temp.

room temp

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Bouin′s solution is a noncoagulant yellow stain fixative. It imposes shrinkage on tissues when used as preservative due to its picric acid component. It is majorly used in ureteroscopic biopsies. It preserves morphological and nuclear information in tissues upon fixation.

Application

Bouin′s solution has been used:
  • in the staining of human liver biopsy tissue samples for two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) imaging
  • in the fixation of rat ovaries for the observing follicle cells using microscopy
  • in the fixation of gonads of crab for morphological and histological analysis

Prepared with saturated picric acid, formaldehyde and acetic acid. Excellent fixative for preserving soft and delicate structures. Used as a mordant in various trichrome procedures.

Pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 2 - STOT SE 3

Target Organs

Eyes,Central nervous system, Respiratory system

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Maternal undernutrition significantly impacts ovarian follicle number and increases ovarian oxidative stress in adult rat offspring
Bernal AB, et al.
Testing, 5(12), e15558-e15558 (2010)
Testis Maturation Stages of Mud Crab (Scylla olivacea) Broodstock on Different Diets
Ikhwanuddin M, et al.
Sains Malaysiana, 47(3), 427-432 (2018)
Nonlinear optical microscopy: use of second harmonic generation and two-photon microscopy for automated quantitative liver fibrosis studies
Sun W, et al.
Journal of Biomedical Optics, 13(6), 064010-064010 (2008)
Improving the interpretation of ureteroscopic biopsies: use of Bouin's fixative
Bultitude MF, et al.
BJU International, 108(9), 1373-1375 (2011)
DNA and RNA obtained from Bouin?s fixed tissues
Bonin S, et al.
Journal of Clinical Pathology, 58(3), 313-316 (2005)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service