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Key Documents

Safety Information

HSANGERG

Sigma-Aldrich

Sanger Arrayed Whole Genome Lentiviral CRISPR Library

Human, Glycerol Format

Synonym(s):

Sanger CRISPR Library

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About This Item

UNSPSC Code:
41105904

Quality Level

packaging

pkg of 50 μL (96-well plate)

application(s)

CRISPR

shipped in

dry ice

storage temp.

−70°C

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This Item
A6150A2221SRP6312
biological source

human plasma

biological source

human plasma

biological source

human plasma

biological source

human

assay

≥70% protein basis (biuret)

assay

-

assay

≥95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

Gene Information

human ... ELA2(1991), SERPINA1(5265)

Gene Information

human ... ELA2(1991), ELANE(1991), SERPINA1(5265)

Gene Information

human ... SERPINC1(462)

Gene Information

human ... Serpin A1(5265)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-

form

salt-free, lyophilized powder

form

salt-free, lyophilized powder

form

lyophilized powder

form

lyophilized

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

General description

Two leaders in genome editing, Sigma-Aldrich and the Wellcome Trust Sanger Institute, have joined forces to make the first ever arrayed lentiviral CRISPR knockout libraries. Based upon validated techniques published in the literature, the Sanger CRISPR libraries will put your lab at the forefront of the race to make the next big discovery.

Application

Functional Genomics/Screening /Target Validation

Features and Benefits

  • Vector: U6-gRNA/PGK-Puro-2A-BFP (gRNA only)
  • Simplify the workflow with puromycin selection
  • Illuminate CRISPR-expressing cells with BFP
  • Flip the expression components into and out of the genome using transposase

Additional Features
  • Better, not bigger: Two optimized clones per human gene reduces the time, cost, and scale of screening experiments
  • Ready-to-screen: Clones are arrayed in a robotics-friendly 96-well format for high throughput screening; requires plasmid preparation to transfect cells or lentivirus preparation to transduce cells
  • Collaborative: Real-time, library validation continues

For detailed information on the Sanger library, click here

Packaging

96-well plates

Components

Bacteria glycerol stock of gRNA-only 3rd generation lenti-based plasmid vector. 2 knockout clones for every human protein-coding gene. Nearly 40,000 sequence confirmed clones


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Physical form

50 μl of bacterial glycerol stocks, 383×96-well plates

Other Notes

This product is for R&D use only, not for drug, household, or other uses. Though the lentiviral transduction particles produced are replication incompetent, it is recommended that they be treated as Risk Group Level 2 (RGL-2) organisms in laboratory handling.

Recommended products

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

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Certificates of Analysis (COA)

Lot/Batch Number

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Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

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Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries.
Metzakopian, E. et al.
Scientific Reports, 7 (2017)

Articles

Genome-wide screening with optimized gRNAs per gene ensures specific and efficient knockout, controlling time and cost.

Protocols

Learn about Sanger Sequencing steps or the chain termination method and how DNA sequencing works and how to read Sanger Sequencing results accurately for your research.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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