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HPA036539

Sigma-Aldrich

Anti-HARS antibody produced in rabbit

enhanced validation

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

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Synonym(s):
Anti-Histidyl-tRNA synthηse
UNSPSC Code:
12352203
Human Protein Atlas Number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

product line

Prestige Antibodies® Powered by Atlas Antibodies

form

buffered aqueous glycerol solution

species reactivity

mouse, rat, human

enhanced validation

RNAi knockdown
Learn more about Antibody Enhanced Validation

technique(s)

immunoblotting: 0.04-0.4 μg/mL
immunofluorescence: 0.25-2 μg/mL
immunohistochemistry: 1:500-1:1000

immunogen sequence

MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDESKQKFVLKTPKGTRD

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... HARS(3035)

General description

The gene encoding histidyl-tRNA synthetase (HARS) enzyme is located on human chromosome 5q31.3. The enzyme HARS belongs to class II aminoacyl tRNA synthetase and catalyses the attachment of the histidine to its cognate transfer RNA (tRNA). It has a catalytic and anticodon binding domain.

Immunogen

histidyl-tRNA synthetase recombinant protein epitope signature tag (PrEST)

Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information.

Biochem/physiol Actions

Histidyl-tRNA synthetase (HARS) interacts with the elongation factor 1 complex and assists in protein biosynthesis. Variants of HARS enzyme is associated with Usher syndrome. Mutations in HARS gene is implicated in peripheral and motor neuron impairment. Mutation in HARS is also implicated in the neurological disorder charcot-marie-tooth disease.

Features and Benefits

Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:
  • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
  • Protein array of 364 human recombinant protein fragments.

Linkage

Corresponding Antigen APREST80080

Physical form

Solution in phosphate buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide.

Legal Information

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A Loss-of-Function Variant in the Human Histidyl-t RNA Synthetase (HARS) Gene is Neurotoxic In Vivo
Vester A, et al.
Human Mutation, 34(1), 191-199 (2013)
Interaction network of human aminoacyl-tRNA synthetases and subunits of elongation factor 1 complex
Lee JS, et al.
Biochemical and Biophysical Research Communications, 291(1), 158-164 (2002)
Genetic mapping and exome sequencing identify variants associated with five novel diseases
Puffenberger EG, et al.
PLoS ONE, 7(1), e28936-e28936 (2012)
Loss of function mutations in HARS cause a spectrum of inherited peripheral neuropathies
Safka B, et al.
Brain, 138(8), 2161-2172 (2015)
Genomic organization, transcriptional mapping, and evolutionary implications of the human bi-directional histidyl-tRNA synthetase locus (HARS/HARSL)
O'Hanlon TP and Miller FW
Biochemical and Biophysical Research Communications, 294(3), 609-614 (2002)

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