Anti-TRIM65 antibody produced in rabbit

Tripartite motif 65 (TRIM65) belongs to TRIM family proteins. TRIM family contains B-box 1 and a B-box 2 type domain. The coiled domain possesses hyper-helical regions. TRIM65 exhibits a SPRY (spla and ryanodine) domain at its C-terminus. Two anti-parallel β-sheets in the SPRY domain form a curved β-sandwich. Trim65 homologous genes have been found in mammals.

Tripartite motif-containing protein 65 recombinant protein epitope signature tag (PrEST)

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Ubiquitination of TNRC6 proteins is through E3 ligase activity of TRIM65. The coiled coil domain plays a role in protein-protein interactions and oligomerization. It is associated with repression of miRNA activity. TRIM65 colludes and adjoins with trinucleotide repeat TNRC6 (trinucleotide repeat-containing gene 6) proteins. Genomic studies on cerebral white matter lesions have found a new locus on chromosome 17q25 accommodating TRIM65. The studies also determine a probable mechanism leading to these lesions. Uncontrolled TRIM65 activities extirpate TNRC6 stability and alter RISC (RNA-induced silencing complex) stability, miRNA activity and cellular homeostasis. Gene silencing of TRIM65 with that of siRNA leads to attenuation of let-7a reporter activity. An abnormal expression of TRIM65 has a similar effect.

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Corresponding Antigen APREST75679

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

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