Anti-USP25 antibody produced in rabbit

The gene USP25 (ubiquitin-specific-processing protease 25) has been mapped to human chromosome 21q11.2. It contains three ubiquitin binding domains at the amino-terminus: one ubiquitin-associated domain and two ubiquitin-interacting motifs. USP25 encodes three different protein isoforms produced by alternate splicing: two are expressed ubiquitously and the longest is restricted to muscle tissues. USP25 is mainly present in the cytoplasm.

Ubiquitin carboxyl-terminal hydrolase 25 recombinant protein epitope signature tag (PrEST)

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies^® protocols and other useful information.

Ubiquitin-specific-processing protease 25 (USP25) is a specific-ubiquitin protease gene. SUMOylation of USP25 impairs its activity by reducing the affinity to ubiquitin chains. In muscle tissues, USP25 is up-regulated during myogenesis. USP25 interacts with myosisn binding protein C1 and protects the latter from degradation which is important in muscle differentiation and maintenance. USP25 is a negative regulator of the virus-triggered type I interferon (IFN) signalling pathway. RT-PCR analysis showed up-regulation of USP25 in Down′s syndrome fetal brains in mouse model. USP25 is down-regulated in human lung cancer. MiR-200c exerts tumor-suppressive effects of non-small-cell lung cancer though suppression of USP25 expression.

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Corresponding Antigen APREST73924

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

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