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Safety Information

HPA002807

Sigma-Aldrich

Anti-MFGE8 antibody produced in rabbit

enhanced validation

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

MFG-E8 Antibody - Anti-MFGE8 antibody produced in rabbit, Mfg-E8 Antibody, Anti-Breast epithelial antigen BA46 antibody produced in rabbit, Anti-HMFG antibody produced in rabbit, Anti-Lactadherin precursor antibody produced in rabbit, Anti-MFG-E8 antibody produced in rabbit, Anti-MFGM antibody produced in rabbit, Anti-Milk fat globule-EGF factor 8 antibody produced in rabbit

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About This Item

UNSPSC Code:
12352203
Human Protein Atlas Number:
HPA002807

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

product line

Prestige Antibodies® Powered by Atlas Antibodies

form

buffered aqueous glycerol solution

species reactivity

human

enhanced validation

orthogonal RNAseq
recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

immunoblotting: 0.04-0.4 μg/mL
immunofluorescence: 0.25-2 μg/mL
immunohistochemistry: 1:500-1:1000

immunogen sequence

LKNNSIPDKQITASSSYKTWGLHLFSWNPSYARLDKQGNFNAWVAGSYGNDQWLQVDLGSSKEVTGIITQGARNFGSVQFVASYKVAYSNDSANWTEYQDPRTGSSKIFPGNWDNHSHKKNLFETPILARYVRILPVAWHNRIALRLEL

UniProt accession no.

Q08431

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MFGE8(4240)

General description

MFGE8 (milk fat globule-EGF factor 8 protein) is a ~53kDa plasma membrane glycoprotein that is generated at the bud from the apical surface of mammary epithelial cells during lactation. It is composed of two specific functional domains: an N-terminal domain with two epidermal growth factor (EGF) repeats and a C-terminal domain with two Discoidin/F5/8C domains for binding of anionic phospholipids and/or extracellular matrices.
MFGE8 is mapped to human chromosome 15q26.1.

Immunogen

Lactadherin precursor recombinant protein epitope signature tag (PrEST)

Application

Anti-MFGE8 antibody produced in rabbit has been used in immunofluorescence.
Anti-MFGE8 antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.

Biochem/physiol Actions

MFGE8 (milk fat globule-EGF factor 8) protein is involved in wide range of cellular interactions such as phagocytosis of apoptotic lymphocytes and other apoptotic cells, cell adhesion of sperm and the egg coat, intestinal mucosa repair, mammary gland branching morphogenesis, angiogenesis etc. It is potentially involved in different stages of sperm maturation and fertilization mechanism. It accelerates the engulfment of apoptotic carcinoma cells by living carcinoma cells which in turn promotes tumor growth.

Features and Benefits

Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:
  • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
  • Protein array of 364 human recombinant protein fragments.

Linkage

Corresponding Antigen APREST77458

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Legal Information

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
B27984
R03939
R003939

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Timothy A Reinhardt et al.
Journal of proteomics, 82, 141-154 (2013-03-06)
Milk protein expression in healthy cows and cows with mastitis will provide information important for the dairy food industry and immune function in the mammary gland. To facilitate protein discovery, milk was fractioned into whey, milk fat globule membranes (MFGM)
Benedikt Kirchner et al.
PloS one, 15(2), e0229606-e0229606 (2020-02-29)
Extracellular vesicles (EVs) such as exosomes are key regulators of intercellular communication that can be found in almost all bio fluids. Although studies in the last decade have made great headway in discerning the role of EVs in many physiological
Manabu Yamazaki et al.
Laboratory investigation; a journal of technical methods and pathology, 94(11), 1260-1272 (2014-09-30)
Milk fat globule--epidermal growth factor (EGF)--factor VIII (MFG-E8) is a secreted glycoprotein that promotes clearance of apoptotic cells by bridging phosphatidylserine on apoptotic cells and integrin αvβ3/5 on phagocytes. High expression of MFG-E8 has been reported in various types of
Mara D Saenz-de-Juano et al.
BMC genomics, 23(1), 189-189 (2022-03-09)
Subclinical mastitis, the inflammation of the mammary gland lacking clinical symptoms, is one of the most prevalent and costly diseases in dairy farming worldwide. Milk microRNAs (miRNAs) encapsulated in extracellular vesicles (EVs) have been proposed as potential biomarkers of different
Mutation Identification for Epilepsy in the US Hispanic Population Using Whole-Exome-Sequencing
Villanos M, et al.
Journal of Cell Biology and Cell Metabolism, 3(011) (2016)
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