HPA002689
Anti-WASHC1 antibody produced in rabbit
Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
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All Photos(7)
Synonym(s):
Anti-FAM39E, Anti-FLJ00038, Anti-WASH1
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biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
product line
Prestige Antibodies® Powered by Atlas Antibodies
form
buffered aqueous glycerol solution
species reactivity
human
enhanced validation
RNAi knockdown
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technique(s)
immunoblotting: 0.04-0.4 μg/mL
immunofluorescence: 0.25-2 μg/mL
immunohistochemistry: 1:1000-1:2500
immunogen sequence
GAPREVVDPSGGWATLLESIRQAGGIGKAKLRSMKERKLEKKKQKEQEQVRATSQGGHLMSDLFNKLVMRHKGISGKGPGAGEGPGGAFARVSDSIPPLPPPQQPQVDEDEDDWES
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... WASH1(100287171)
General description
WASH complex subunit 1 (WASHC1) is located on human chromosome 9p24.3. It belongs to Wiskott-Aldrich Syndrome Protein and SCAR Homolog (WASH) family. WASHC1 protein has an actin binding domain.
Immunogen
WASH complex subunit 1 recombinant protein epitope signature tag (PrEST)
Application
Anti-WASHC1 antibody produced in rabbit has been used:
- in the detection of WASH complex in Dictyostelium cells by immunofluorescence imaging.
- in the detection of WASH proteins in HeLa cells using confocal microscopy.
- in western blotting for the detection of WASH1 complexes in human leukemic Jurkat T cells.
Biochem/physiol Actions
WASH exists as multiple complex protein in HeLa and bovine cells. WASH proteins coordinate the migration of endosomal α5β1 integrin. WASHC1 protein regulates actin cytoskeleton organisation. WASH1 protein is regarded as actin-nucleating promoting factor. WASH protein complex interacts with actin related protein, Arp2/3 and mediates actin networking.
Features and Benefits
Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.
Every Prestige Antibody is tested in the following ways:
Every Prestige Antibody is tested in the following ways:
- IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
- Protein array of 364 human recombinant protein fragments.
Linkage
Corresponding Antigen APREST74318
Physical form
Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide
Legal Information
Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Human subtelomeric WASH genes encode a new subclass of the WASP family
Linardopoulou EV, et al.
PLoS Genetics, 3(12), e237-e237 (2007)
The Arp2/3 activator WASH regulates alpha5beta1-integrin-mediated invasive migration
Zech T, et al.
Journal of Cell Science, 124(22), 3753-3759 (2011)
Catherine M Buckley et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(40), E5906-E5915 (2016-09-21)
Macropinocytosis is an ancient mechanism that allows cells to harvest nutrients from extracellular media, which also allows immune cells to sample antigens from their surroundings. During macropinosome formation, bulk plasma membrane is internalized with all its integral proteins. It is
Functional characterization of Wiskott Aldrich Syndrome protein and scar homolog (WASH), a bi-modular nucleation promoting factor (NPF) able to interact with biogenesis of lysosome related organelle subunit 2 (BLOS-2) and gamma-tubulin
Monfregola J, et al.
The Journal of Biological Chemistry, jbc-M109 (2010)
Sorting nexin 27 interactome in T-lymphocytes identifies zona occludens-2 dynamic redistribution at the immune synapse
Tello-Lafoz M, et al.
Traffic, 18(8), 491-504 (2017)
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