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Merck
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HPA000232

Anti-GATA1 antibody produced in rabbit

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

Anti-ERYF1, Anti-GATA-1, Anti-GF1, Anti-NF-E1, Anti-NFE1

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About This Item

UNSPSC Code:
12352203
Human Protein Atlas Number:
HPA000232
NACRES:
NA.43
MDL number:
MFCD02096392

Key Documents

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Product Name

Anti-GATA1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

product line

Prestige Antibodies® Powered by Atlas Antibodies

form

buffered aqueous glycerol solution

species reactivity

human

enhanced validation

orthogonal RNAseq
recombinant expression
Learn more about Antibody Enhanced Validation

Quality Level

100

Gene Information

human ... GATA1(2623)

technique(s)

ChIP: 1-10 μg (per reaction)
immunoblotting: 0.04-0.4 μg/mL
immunohistochemistry: 1:20-1:50

immunogen sequence

EGLDAAASSTAPSTATAAAAALAYYRDAEAYRHSPVFQVYPLLNCMEGIPGGSPYAGWAYGKTGLYPASTVCPTREDSPPQAVEDLDGKGSTSFLETLKTERLSPDLLTLGPALP

UniProt accession no.

P15976

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

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Features and Benefits

Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:
  • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
  • Protein array of 364 human recombinant protein fragments.

Immunogen

Erythroid transcription factor recombinant protein epitope signature tag (PrEST)

Other Notes

Corresponding Antigen APREST74059

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Legal Information

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

Application

Anti-GATA1 antibody produced in rabbit is suitable for:
  • immunohistochemistry (formalin-fixed, paraffin-embedded sections)
  • indirect immunofluorescence
  • western blot

Anti-GATA1 antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.

Biochem/physiol Actions

Erythroid transcription factor (GATA1) is a transcriptional activator or repressor which serves as a universal alteration factor for erythroid development. It binds to DNA sites with the consensus sequence 5′-[AT]GATA[AG]-3′ within regulatory regions of globin genes and of other genes expressed in erythroid cells.

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
R00146
R000146

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Yan Zeng et al.
PloS one, 7(1), e29518-e29518 (2012-01-12)
The human ZNF268 gene encodes a typical KRAB-C2H2 zinc finger protein that may participate in hematopoiesis and leukemogenesis. A recent microarray study revealed that ZNF268 expression continuously decreases during erythropoiesis. However, the molecular mechanisms underlying regulation of ZNF268 during hematopoiesis

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